Biomaterial Database

Introducing sorghum DREB2 gene in maize (Zea mays L.) to improve drought and salinity tolerance. 2023

Ali Izadi-Darbandi, and Hussien Alameldin, and Nima Namjoo, and Khalil Ahmad

Department of Agronomy and Plant Breeding Sciences, College of Aburaihan, University of Tehran, Tehran, Iran.

Salinity and drought are significant abiotic stresses causing a considerable loss of seed and biomass yield in most commercial crops. Some of the most critical players in the abscisic acid pathway are drought responsive element binding (DREB) proteins that are a part of AP2/ethylene response factor transcription factors that bind to promoters of some family genes needed to be expressed under abiotic stresses. In this study, salt- and drought-tolerant maize plants were produced from immature maize embryos bombarded by the sorghum (Sorghum bicolor L.) DREB2 gene that is linked to hygromycin resistance (hpt) genes. The putative transgenic calli were transferred to an N6 medium containing 1 mg/L benzylaminopurine and 50 mg/L hygromycin. Regeneration was completed after 4 weeks on selective media under a 16/8 h light/dark condition at 25°C. Polymerase chain reaction (PCR) and reverse transcription-PCR approved the existence of upstream promoter (rd29a), hpt gene, and the expression of the DREB2 in transgenes up to the third generation (T2). It was found that the K+/Na+ ratio and the amount of proline as a screening indicator were higher in transgenic plants compared to their wild types. This result is a promising model to enhance maize tolerance to abiotic stressors.

UI	MeSH Term	Description	Entries
D010940	Plant Proteins	Proteins found in plants (flowers, herbs, shrubs, trees, etc.). The concept does not include proteins found in vegetables for which PLANT PROTEINS, DIETARY is available.	Plant Protein,Protein, Plant,Proteins, Plant
D003313	Zea mays	A plant species of the family POACEAE. It is a tall grass grown for its EDIBLE GRAIN, corn, used as food and animal FODDER.	Corn,Indian Corn,Maize,Teosinte,Zea,Corn, Indian
D013312	Stress, Physiological	The unfavorable effect of environmental factors (stressors) on the physiological functions of an organism. Prolonged unresolved physiological stress can affect HOMEOSTASIS of the organism, and may lead to damaging or pathological conditions.	Biotic Stress,Metabolic Stress,Physiological Stress,Abiotic Stress,Abiotic Stress Reaction,Abiotic Stress Response,Biological Stress,Metabolic Stress Response,Physiological Stress Reaction,Physiological Stress Reactivity,Physiological Stress Response,Abiotic Stress Reactions,Abiotic Stress Responses,Abiotic Stresses,Biological Stresses,Biotic Stresses,Metabolic Stress Responses,Metabolic Stresses,Physiological Stress Reactions,Physiological Stress Responses,Physiological Stresses,Reaction, Abiotic Stress,Reactions, Abiotic Stress,Response, Abiotic Stress,Response, Metabolic Stress,Stress Reaction, Physiological,Stress Response, Metabolic,Stress Response, Physiological,Stress, Abiotic,Stress, Biological,Stress, Biotic,Stress, Metabolic
D045868	Sorghum	A plant genus of the family POACEAE. The grain is used for FOOD and for ANIMAL FEED. This should not be confused with KAFFIR LIME or with KEFIR milk product.	Johnsongrass,Kaffir Corn,Kafir,Sorghum bicolor,Sorghum halepense,Sudangrass,Corn, Kaffir,Corns, Kaffir,Johnsongrasses,Kaffir Corns,Kafirs,Sorghum bicolors,Sorghum halepenses,Sorghums,Sudangrasses,bicolors, Sorghum,halepenses, Sorghum
D055049	Salt Tolerance	The ability of organisms to sense and adapt to high concentrations of salt in their growth environment.	Salt-Tolerance,Saline-Tolerance,Salinity Tolerance,Salt Adaptation,Salt Adaption,Salt-Adaption,Adaptation, Salt,Adaption, Salt,Saline Tolerance,Salinity Tolerances,Salt Adaptations,Salt Adaptions,Salt Tolerances,Tolerance, Salinity,Tolerance, Salt
D055864	Droughts	Prolonged dry periods in natural climate cycle. They are slow-onset phenomena caused by rainfall deficit combined with other predisposing factors.	Drought
D018506	Gene Expression Regulation, Plant	Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in plants.	Plant Gene Expression Regulation,Regulation of Gene Expression, Plant,Regulation, Gene Expression, Plant
D030821	Plants, Genetically Modified	PLANTS, or their progeny, whose GENOME has been altered by GENETIC ENGINEERING.	Genetically Modified Plants,Plants, Transgenic,Transgenic Plants,GMO Plants,Genetically Engineered Plants,Engineered Plant, Genetically,Engineered Plants, Genetically,GMO Plant,Genetically Engineered Plant,Genetically Modified Plant,Modified Plant, Genetically,Modified Plants, Genetically,Plant, GMO,Plant, Genetically Engineered,Plant, Genetically Modified,Plant, Transgenic,Plants, GMO,Plants, Genetically Engineered,Transgenic Plant