-transparent.png){kind=logo}
Defective lambda transducing phages for the nalA region of the Escherichia coli chromosome were isolated from a lysogen in which lambda is inserted in the nearby glpT gene. The three classes of transducing phages designated lambdanrdA, lambdaubiG, and lambdadnalA contained bacterial DNA extending from glpT through nrdA, ubiG, and nalA, respectively. The bacterial genes are in the left arm of the lambda chromosome. Of the eleven polypeptides coded by lambdadnalA that were resolved by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate only one was not also specified by lambdadubiG. This 105,000 dalton polypeptide is the nalA gene product. The electorphoretic mobility and isoelectric point of this protein were unaffected by a nalA mutation (nalA48) that confers nalidixic acid resistance. Temperature-sensitive and amber mutations in the nalA gene were isolated using a lambdadnalA48 lysogen which is heterodiploid for nalA. The conditional lethality of these mutations proves that nalA is an essential locus.
K N Kreuzer, and
K McEntee, and
A P Geballe, and
N R Cozzarelli
January 1981,
Genetika,
K N Kreuzer, and
K McEntee, and
A P Geballe, and
N R Cozzarelli
June 1971,
Journal of molecular biology,
K N Kreuzer, and
K McEntee, and
A P Geballe, and
N R Cozzarelli
January 1980,
Molecular & general genetics : MGG,
K N Kreuzer, and
K McEntee, and
A P Geballe, and
N R Cozzarelli
July 1978,
Molecular & general genetics : MGG,
K N Kreuzer, and
K McEntee, and
A P Geballe, and
N R Cozzarelli
January 1980,
Molecular & general genetics : MGG,
K N Kreuzer, and
K McEntee, and
A P Geballe, and
N R Cozzarelli
April 1978,
Molecular & general genetics : MGG,
K N Kreuzer, and
K McEntee, and
A P Geballe, and
N R Cozzarelli
January 2008,
Methods in molecular biology (Clifton, N.J.),
K N Kreuzer, and
K McEntee, and
A P Geballe, and
N R Cozzarelli
May 2004,
Journal of bacteriology,
K N Kreuzer, and
K McEntee, and
A P Geballe, and
N R Cozzarelli
January 1975,
Proceedings of the National Academy of Sciences of the United States of America,
K N Kreuzer, and
K McEntee, and
A P Geballe, and
N R Cozzarelli
January 1986,
Methods in enzymology,
