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Potential acetylcholine receptor (AChR) mutants of the nematode are selectable by resistance to the neurotoxic drug levamisole, a probable cholinergic agonist. To determine which mutants may have achieved resistance through loss of levamisole receptor function, we have assayed mutant extracts for specific 3H-meta-aminolevamisole binding activity in the presence and absence of mecamylamine. We find that mutants in 3 of the 7 genes associated with extreme levamisole resistance are obviously deficient in saturable specific 3H-meta-aminolevamisole binding activity. Mutants of the 4 other genes have abnormal binding activities that fail to undergo the apparent allosteric activation of saturable specific 3H-meta-aminolevamisole binding activity caused by mecamylamine. Thus, all 7 genes appear to be required to produce a fully functional levamisole receptor. Mutants of several other genes associated only with partial resistance to levamisole have at least grossly normal receptor binding activities.
J A Lewis, and
J S Elmer, and
J Skimming, and
S McLafferty, and
J Fleming, and
T McGee
February 1987,
Molecular pharmacology,
J A Lewis, and
J S Elmer, and
J Skimming, and
S McLafferty, and
J Fleming, and
T McGee
January 1979,
Genetics,
J A Lewis, and
J S Elmer, and
J Skimming, and
S McLafferty, and
J Fleming, and
T McGee
January 1977,
Molecular & general genetics : MGG,
J A Lewis, and
J S Elmer, and
J Skimming, and
S McLafferty, and
J Fleming, and
T McGee
October 1984,
Science (New York, N.Y.),
J A Lewis, and
J S Elmer, and
J Skimming, and
S McLafferty, and
J Fleming, and
T McGee
June 1975,
Genetics,
J A Lewis, and
J S Elmer, and
J Skimming, and
S McLafferty, and
J Fleming, and
T McGee
January 1980,
Molecular & general genetics : MGG,
J A Lewis, and
J S Elmer, and
J Skimming, and
S McLafferty, and
J Fleming, and
T McGee
March 1986,
Genetics,
J A Lewis, and
J S Elmer, and
J Skimming, and
S McLafferty, and
J Fleming, and
T McGee
February 1984,
Genetics,
J A Lewis, and
J S Elmer, and
J Skimming, and
S McLafferty, and
J Fleming, and
T McGee
October 1978,
Genetics,
J A Lewis, and
J S Elmer, and
J Skimming, and
S McLafferty, and
J Fleming, and
T McGee
April 1985,
Journal of neurogenetics,
