Effects of Oxidative Stress on the Autophagy and Apoptosis of Granulosa Cells in Broody Geese. 2023

Li'E Hou, and Tiantian Gu, and Kaiqi Weng, and Yu Zhang, and Yang Zhang, and Guohong Chen, and Qi Xu
Jiangsu Key Laboratory for Animal Genetic, Breeding, and Molecular Design, Yangzhou University, Yangzhou 225009, China.

Broodiness is an unfavorable trait associated with the cessation of egg laying. Studies have found that excessive granulosa cell apoptosis and autophagy occur during goose broodiness. Other studies have also confirmed that oxidative stress is an important cause of apoptosis and autophagy. However, whether oxidative stress occurs during goose broodiness and whether this oxidative stress causes apoptosis and autophagy have not been fully elucidated. In this study, we investigated the effects of oxidative stress on the autophagy and apoptosis of granulosa cells in broody geese. The results showed higher mRNA expression of genes related to antioxidative stress responses (GPX, SOD-1, SOD-2, COX-2, CAT and hsp70) in pre-broody and broody geese than in laying birds. In addition, increased levels of granulosa cell apoptosis and autophagy were observed in pre-broody geese than in laying geese. Additionally, granulosa cells treated with H2O2 exhibited increased apoptosis and autophagy in vitro, and these effects were responsible for goose granulosa cell death. Moreover, vitamin E treatment effectively protected granulosa cells from H2O2-induced oxidative stress by inhibiting ROS production. Correspondingly, granulosa cell apoptosis and autophagy were greatly alleviated by vitamin E treatment. Together, our results demonstrated serious oxidative stress and granulosa cell apoptosis and autophagy in broody geese, and oxidative stress promoted apoptosis and autophagy. Vitamin E alleviated the autophagy and apoptosis of granulosa cells by inhibiting oxidative stress.

UI MeSH Term Description Entries
D005260 Female Females
D005777 Geese Any of various large waterfowl in the order Anseriformes, especially those of the genera Anser (gray geese) and Branta (black geese). They are larger than ducks but smaller than swans, prefer FRESH WATER, and occur primarily in the northern hemisphere. Goose,Gooses
D006107 Granulosa Cells Supporting cells for the developing female gamete in the OVARY. They are derived from the coelomic epithelial cells of the gonadal ridge. Granulosa cells form a single layer around the OOCYTE in the primordial ovarian follicle and advance to form a multilayered cumulus oophorus surrounding the OVUM in the Graafian follicle. The major functions of granulosa cells include the production of steroids and LH receptors (RECEPTORS, LH). Cell, Granulosa,Cells, Granulosa,Granulosa Cell
D006861 Hydrogen Peroxide A strong oxidizing agent used in aqueous solution as a ripening agent, bleach, and topical anti-infective. It is relatively unstable and solutions deteriorate over time unless stabilized by the addition of acetanilide or similar organic materials. Hydrogen Peroxide (H2O2),Hydroperoxide,Oxydol,Perhydrol,Superoxol,Peroxide, Hydrogen
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D001343 Autophagy The segregation and degradation of various cytoplasmic constituents via engulfment by MULTIVESICULAR BODIES; VACUOLES; or AUTOPHAGOSOMES and their digestion by LYSOSOMES. It plays an important role in BIOLOGICAL METAMORPHOSIS and in the removal of bone by OSTEOCLASTS. Defective autophagy is associated with various diseases, including NEURODEGENERATIVE DISEASES and cancer. Autophagocytosis,ER-Phagy,Lipophagy,Nucleophagy,Reticulophagy,Ribophagy,Autophagy, Cellular,Cellular Autophagy,ER Phagy
D017209 Apoptosis A regulated cell death mechanism characterized by distinctive morphologic changes in the nucleus and cytoplasm, including the endonucleolytic cleavage of genomic DNA, at regularly spaced, internucleosomal sites, i.e., DNA FRAGMENTATION. It is genetically programmed and serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth. Apoptosis, Extrinsic Pathway,Apoptosis, Intrinsic Pathway,Caspase-Dependent Apoptosis,Classic Apoptosis,Classical Apoptosis,Programmed Cell Death,Programmed Cell Death, Type I,Apoptoses, Extrinsic Pathway,Apoptoses, Intrinsic Pathway,Apoptosis, Caspase-Dependent,Apoptosis, Classic,Apoptosis, Classical,Caspase Dependent Apoptosis,Cell Death, Programmed,Classic Apoptoses,Extrinsic Pathway Apoptoses,Extrinsic Pathway Apoptosis,Intrinsic Pathway Apoptoses,Intrinsic Pathway Apoptosis
D018384 Oxidative Stress A disturbance in the prooxidant-antioxidant balance in favor of the former, leading to potential damage. Indicators of oxidative stress include damaged DNA bases, protein oxidation products, and lipid peroxidation products (Sies, Oxidative Stress, 1991, pxv-xvi). Anti-oxidative Stress,Antioxidative Stress,DNA Oxidative Damage,Nitro-Oxidative Stress,Oxidative Cleavage,Oxidative DNA Damage,Oxidative Damage,Oxidative Injury,Oxidative Nitrative Stress,Oxidative Stress Injury,Oxidative and Nitrosative Stress,Stress, Oxidative,Anti oxidative Stress,Anti-oxidative Stresses,Antioxidative Stresses,Cleavage, Oxidative,DNA Damage, Oxidative,DNA Oxidative Damages,Damage, DNA Oxidative,Damage, Oxidative,Damage, Oxidative DNA,Injury, Oxidative,Injury, Oxidative Stress,Nitrative Stress, Oxidative,Nitro Oxidative Stress,Nitro-Oxidative Stresses,Oxidative Cleavages,Oxidative DNA Damages,Oxidative Damage, DNA,Oxidative Damages,Oxidative Injuries,Oxidative Nitrative Stresses,Oxidative Stress Injuries,Oxidative Stresses,Stress Injury, Oxidative,Stress, Anti-oxidative,Stress, Antioxidative,Stress, Nitro-Oxidative,Stress, Oxidative Nitrative,Stresses, Nitro-Oxidative

Related Publications

Li'E Hou, and Tiantian Gu, and Kaiqi Weng, and Yu Zhang, and Yang Zhang, and Guohong Chen, and Qi Xu
September 2023, Poultry science,
Li'E Hou, and Tiantian Gu, and Kaiqi Weng, and Yu Zhang, and Yang Zhang, and Guohong Chen, and Qi Xu
March 2024, British poultry science,
Li'E Hou, and Tiantian Gu, and Kaiqi Weng, and Yu Zhang, and Yang Zhang, and Guohong Chen, and Qi Xu
December 2020, Chemosphere,
Li'E Hou, and Tiantian Gu, and Kaiqi Weng, and Yu Zhang, and Yang Zhang, and Guohong Chen, and Qi Xu
May 2024, International journal of molecular sciences,
Li'E Hou, and Tiantian Gu, and Kaiqi Weng, and Yu Zhang, and Yang Zhang, and Guohong Chen, and Qi Xu
October 2018, Bioscience reports,
Li'E Hou, and Tiantian Gu, and Kaiqi Weng, and Yu Zhang, and Yang Zhang, and Guohong Chen, and Qi Xu
August 2021, Reproductive sciences (Thousand Oaks, Calif.),
Li'E Hou, and Tiantian Gu, and Kaiqi Weng, and Yu Zhang, and Yang Zhang, and Guohong Chen, and Qi Xu
February 2024, Theriogenology,
Li'E Hou, and Tiantian Gu, and Kaiqi Weng, and Yu Zhang, and Yang Zhang, and Guohong Chen, and Qi Xu
September 2022, Ecotoxicology and environmental safety,
Li'E Hou, and Tiantian Gu, and Kaiqi Weng, and Yu Zhang, and Yang Zhang, and Guohong Chen, and Qi Xu
January 2023, Journal of animal science,
Li'E Hou, and Tiantian Gu, and Kaiqi Weng, and Yu Zhang, and Yang Zhang, and Guohong Chen, and Qi Xu
February 2022, Toxicology,
Copied contents to your clipboard!