-transparent.png){kind=logo}
The immunological detection of soluble pneumococcal polysaccharide antigens in pathological products is of importance in the direct diagnosis of meningitis or pulmonary infections. We have developed a double antibody sandwich ELISA method using a biotin-avidin system using antibodies constituted with a mixture of IgGs from pooled and/or monospecific antipneumococcal sera provided by the Danish Statens Seruminstitut. The sensitivity of this rapid ELISA method was optimized with purified capsular polysaccharides of the 24 main pneumococcal serotypes. With incubation steps of 30 min at 37 degrees C for the antigens and the conjugates, the detection limit was close to 1 ng/ml for 75% of the purified polysaccharides. A retrospective study of 46 CSF samples established the validity of the assay. This type of modified ELISA system represents a specific, sensitive and rapid procedure for the potential detection of capsular soluble antigens of all pneumococcal serotypes.
J M Da Costa Castro, and
F Deschamps, and
M Benbachir, and
J Henrichsen, and
P J Volle, and
R M Guinet
April 2017,
Drug testing and analysis,
J M Da Costa Castro, and
F Deschamps, and
M Benbachir, and
J Henrichsen, and
P J Volle, and
R M Guinet
January 2021,
Methods in molecular biology (Clifton, N.J.),
J M Da Costa Castro, and
F Deschamps, and
M Benbachir, and
J Henrichsen, and
P J Volle, and
R M Guinet
December 2014,
Journal of translational medicine,
J M Da Costa Castro, and
F Deschamps, and
M Benbachir, and
J Henrichsen, and
P J Volle, and
R M Guinet
May 2015,
Biosensors & bioelectronics,
J M Da Costa Castro, and
F Deschamps, and
M Benbachir, and
J Henrichsen, and
P J Volle, and
R M Guinet
January 1979,
Methods in enzymology,
J M Da Costa Castro, and
F Deschamps, and
M Benbachir, and
J Henrichsen, and
P J Volle, and
R M Guinet
May 1987,
Diagnostic microbiology and infectious disease,
J M Da Costa Castro, and
F Deschamps, and
M Benbachir, and
J Henrichsen, and
P J Volle, and
R M Guinet
February 2004,
Journal of microbiological methods,
J M Da Costa Castro, and
F Deschamps, and
M Benbachir, and
J Henrichsen, and
P J Volle, and
R M Guinet
June 2018,
Analytical chemistry,
J M Da Costa Castro, and
F Deschamps, and
M Benbachir, and
J Henrichsen, and
P J Volle, and
R M Guinet
August 1986,
Journal of immunological methods,
J M Da Costa Castro, and
F Deschamps, and
M Benbachir, and
J Henrichsen, and
P J Volle, and
R M Guinet
November 1983,
International journal of pediatric otorhinolaryngology,
