BIOMAT Database Logo BIOMAT Database Logo

Pneumococcal antigens in sputa: ELISA for the detection of pneumococcal C-polysaccharide in sputa from pneumonia patients. 1987

A Krook, and H Holmberg
Department of Infectious Diseases, Roslagstulls Hospital, Karolinska Institute, Stockholm, Sweden.

An improved ELISA, the LKB UltroBact Pneumococcus Kit detecting pneumococcal C-polysaccharide, has been tested. Sputum samples from 72 patients with community acquired pneumonia were included in the study. The sensitivity obtained was 96.1% and the specificity 92.6%. This ELISA might offer a useful diagnostic method in major clinical microbiologic laboratories for demonstrating Streptococcus pneumonia in sputa from patients with pneumonia.

UI MeSH Term Description Entries
D011018 Pneumonia, Pneumococcal A febrile disease caused by STREPTOCOCCUS PNEUMONIAE. Pneumococcal Pneumonia,Pneumococcal Pneumonias,Pneumonias, Pneumococcal
D011135 Polysaccharides, Bacterial Polysaccharides found in bacteria and in capsules thereof. Bacterial Polysaccharides
D003937 Diagnosis, Differential Determination of which one of two or more diseases or conditions a patient is suffering from by systematically comparing and contrasting results of diagnostic measures. Diagnoses, Differential,Differential Diagnoses,Differential Diagnosis
D004797 Enzyme-Linked Immunosorbent Assay An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed. ELISA,Assay, Enzyme-Linked Immunosorbent,Assays, Enzyme-Linked Immunosorbent,Enzyme Linked Immunosorbent Assay,Enzyme-Linked Immunosorbent Assays,Immunosorbent Assay, Enzyme-Linked,Immunosorbent Assays, Enzyme-Linked
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D013183 Sputum Material coughed up from the lungs and expectorated via the mouth. It contains MUCUS, cellular debris, and microorganisms. It may also contain blood or pus. Sputum, Induced,Induced Sputum,Induced Sputums,Sputums,Sputums, Induced
D013296 Streptococcus pneumoniae A gram-positive organism found in the upper respiratory tract, inflammatory exudates, and various body fluids of normal and/or diseased humans and, rarely, domestic animals. Diplococcus pneumoniae,Pneumococcus

Related Publications

A Krook, and H Holmberg
Rapid detection of pneumococcal antigens in sputa in patients with community-acquired pneumonia by coagglutination.
January 1988, Medical microbiology and immunology,
A Krook, and H Holmberg
Detection of pneumococcal polysaccharide antigens in the urine of patients with bacteraemic and non-bacteraemic pneumococcal pneumonia.
November 1994, Zentralblatt fur Bakteriologie : international journal of medical microbiology,
A Krook, and H Holmberg
Highly sensitive biotin-avidin sandwich ELISA for the rapid detection of pneumococcal capsular polysaccharide antigens.
November 1987, Journal of immunological methods,
A Krook, and H Holmberg
Detection of pneumococcal polysaccharide in the sputum of patients with pneumococcal pneumonia by counterimmunoelectrophoresis.
March 1976, The Journal of laboratory and clinical medicine,
A Krook, and H Holmberg
Quantitation of pneumococcal C polysaccharide in sputum samples from patients with presumptive pneumococcal pneumonia by enzyme immunoassay.
February 1992, Journal of clinical microbiology,
A Krook, and H Holmberg
Pneumococcal polysaccharide antigens: capsules and C-polysaccharide. An immunochemical study.
February 1995, Danish medical bulletin,
A Krook, and H Holmberg
Coagglutination and counterimmunoelectrophoresis for detection of pneumococcal antigens in the sputum of pneumonia patients.
May 1980, Journal of clinical microbiology,
A Krook, and H Holmberg
[Detection of the polysaccharide pneumococcal antigen in CSF by the ELISA method].
January 1985, Revista de igiena, bacteriologie, virusologie, parazitologie, epidemiologie, pneumoftiziologie. Bacteriologia, virusologia, parazitologia, epidemiologia,
A Krook, and H Holmberg
Re.: Anti-pneumococcal polysaccharide ELISA.
April 1986, Journal of immunological methods,
A Krook, and H Holmberg
Detection of penumococcal antigens in the sputum in pneumococcal pneumonia.
November 1977, The American review of respiratory disease,
Copied contents to your clipboard!