Biomaterial Database

Phagocytosis and intracellular killing of Mycobacterium avium complex by human and murine macrophages. 1987

L E Bermudez, and L S Young

Kuzell Institute for Arthritis & Infectious Diseases, Medical Research Institute at Pacific Presbyterian Medical Center, San Francisco, CA 94115.

1. Host defenses against Mycobacterium avium complex (MAC) are poorly defined. Peritoneal macrophages from black and beige mice, and cultured human macrophages were infected in vitro with MAC serotype 1 from an AIDS patient, in the presence or absence of normal or convalescent serum. Bacteria:cell ratio was 1:10. Supernatants and macrophage lysates were cultured 6, 24 and 48 h later to determine the uptake and killing by macrophages. Phagocytosis by activated macrophages, obtained from pre-infected and treated mice or stimulated in vitro with endotoxin, was also studied. 2. Neither convalescent serum nor normal serum caused a significant increase in MAC phagocytosis. 3. Unstimulated macrophages from black or beige mice and humans were incapable of killing the intracellular bacteria. Activated macrophages from all sources phagocytized and killed 80 +/- 4% of the initial inoculum after 48 h in culture. 4. These results demonstrate that activated macrophages are required for optimal intracellular killing of serotype 1 MAC.

UI	MeSH Term	Description	Entries
D008262	Macrophage Activation	The process of altering the morphology and functional activity of macrophages so that they become avidly phagocytic. It is initiated by lymphokines, such as the macrophage activation factor (MAF) and the macrophage migration-inhibitory factor (MMIF), immune complexes, C3b, and various peptides, polysaccharides, and immunologic adjuvants.	Activation, Macrophage,Activations, Macrophage,Macrophage Activations
D008264	Macrophages	The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)	Bone Marrow-Derived Macrophages,Monocyte-Derived Macrophages,Macrophage,Macrophages, Monocyte-Derived,Bone Marrow Derived Macrophages,Bone Marrow-Derived Macrophage,Macrophage, Bone Marrow-Derived,Macrophage, Monocyte-Derived,Macrophages, Bone Marrow-Derived,Macrophages, Monocyte Derived,Monocyte Derived Macrophages,Monocyte-Derived Macrophage
D009162	Mycobacterium avium	A bacterium causing tuberculosis in domestic fowl and other birds. In pigs, it may cause localized and sometimes disseminated disease. The organism occurs occasionally in sheep and cattle. It should be distinguished from the M. avium complex, which infects primarily humans.
D010587	Phagocytosis	The engulfing and degradation of microorganisms; other cells that are dead, dying, or pathogenic; and foreign particles by phagocytic cells (PHAGOCYTES).	Phagocytoses
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D014774	Virulence	The degree of pathogenicity within a group or species of microorganisms or viruses as indicated by case fatality rates and/or the ability of the organism to invade the tissues of the host. The pathogenic capacity of an organism is determined by its VIRULENCE FACTORS.	Pathogenicity
D051379	Mice	The common name for the genus Mus.	Mice, House,Mus,Mus musculus,Mice, Laboratory,Mouse,Mouse, House,Mouse, Laboratory,Mouse, Swiss,Mus domesticus,Mus musculus domesticus,Swiss Mice,House Mice,House Mouse,Laboratory Mice,Laboratory Mouse,Mice, Swiss,Swiss Mouse,domesticus, Mus musculus