Biochemical mechanisms of tributyltin chloride-induced cell toxicity in Sertoli cells. 2023

Pengchen Chen, and Junhui Chen, and Wei Zhang, and Li Tang, and Guangqing Cheng, and Huiying Li, and Tianyun Fan, and Jigang Wang, and Wenbin Zhong, and Yali Song
Dongguan Maternal and Child Health Care Hospital, Postdoctoral Innovation Practice Base of Southern Medical University, Dongguan 523125, Guangdong, China; Department of Nephrology, Shenzhen key Laboratory of Kidney Diseases, and Shenzhen Clinical Research Centre for Geriatrics, Shenzhen People's Hospital (The Second Clinical Medical College, Jinan University, The First Affiliated Hospital, Southern University of Science and Technology), Shenzhen 518020, China.

Tributyltin chloride (TBTCL) is a widely used fungicide and heat stabilizer in compositions of PVC. TBTCL has been detected in human bodies and potentially causes harmful effects on humans' thyroid, cardiovascular and other organs. As one of the first examples of endocrine disruptors, the toxicity effects of TBTCL on the male reproduction system have aroused concerns. However, the potential cellular mechanisms are not fully explored. In the current study, by using Sertoli cells, a critical regulator of spermatogenesis as a cell model, we showed that with 200 nM exposure for 24 h, TBTCL causes apoptosis and cell cycle arrest. RNA sequencing analyses suggested that TBTCL probably activates endoplasmic reticulum (ER) stress, and disrupts autophagy. Biochemical analysis showed that TBTCL indeed induces ER stress and the dysregulation of autophagy. Interestingly, activation of ER stress and inhibition of autophagy is responsible for TBTCL-induced apoptosis and cell cycle arrest. Our results thus uncovered a novel insight into the cellular mechanisms for TBTCL-induced toxicology in Sertoli cells.

UI MeSH Term Description Entries
D008297 Male Males
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D001343 Autophagy The segregation and degradation of various cytoplasmic constituents via engulfment by MULTIVESICULAR BODIES; VACUOLES; or AUTOPHAGOSOMES and their digestion by LYSOSOMES. It plays an important role in BIOLOGICAL METAMORPHOSIS and in the removal of bone by OSTEOCLASTS. Defective autophagy is associated with various diseases, including NEURODEGENERATIVE DISEASES and cancer. Autophagocytosis,ER-Phagy,Lipophagy,Nucleophagy,Reticulophagy,Ribophagy,Autophagy, Cellular,Cellular Autophagy,ER Phagy
D012708 Sertoli Cells Supporting cells projecting inward from the basement membrane of SEMINIFEROUS TUBULES. They surround and nourish the developing male germ cells and secrete the ANDROGEN-BINDING PROTEIN and hormones such as ANTI-MULLERIAN HORMONE. The tight junctions of Sertoli cells with the SPERMATOGONIA and SPERMATOCYTES provide a BLOOD-TESTIS BARRIER. Sertoli Cell,Cell, Sertoli,Cells, Sertoli
D013091 Spermatogenesis The process of germ cell development in the male from the primordial germ cells, through SPERMATOGONIA; SPERMATOCYTES; SPERMATIDS; to the mature haploid SPERMATOZOA. Spermatocytogenesis,Spermiogenesis
D013961 Thyroid Gland A highly vascularized endocrine gland consisting of two lobes joined by a thin band of tissue with one lobe on each side of the TRACHEA. It secretes THYROID HORMONES from the follicular cells and CALCITONIN from the parafollicular cells thereby regulating METABOLISM and CALCIUM level in blood, respectively. Thyroid,Gland, Thyroid,Glands, Thyroid,Thyroid Glands,Thyroids
D014220 Trialkyltin Compounds Organometallic compounds which contain tin and three alkyl groups. Compounds, Trialkyltin
D017209 Apoptosis A regulated cell death mechanism characterized by distinctive morphologic changes in the nucleus and cytoplasm, including the endonucleolytic cleavage of genomic DNA, at regularly spaced, internucleosomal sites, i.e., DNA FRAGMENTATION. It is genetically programmed and serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth. Apoptosis, Extrinsic Pathway,Apoptosis, Intrinsic Pathway,Caspase-Dependent Apoptosis,Classic Apoptosis,Classical Apoptosis,Programmed Cell Death,Programmed Cell Death, Type I,Apoptoses, Extrinsic Pathway,Apoptoses, Intrinsic Pathway,Apoptosis, Caspase-Dependent,Apoptosis, Classic,Apoptosis, Classical,Caspase Dependent Apoptosis,Cell Death, Programmed,Classic Apoptoses,Extrinsic Pathway Apoptoses,Extrinsic Pathway Apoptosis,Intrinsic Pathway Apoptoses,Intrinsic Pathway Apoptosis
D059865 Endoplasmic Reticulum Stress Various physiological or molecular disturbances that impair ENDOPLASMIC RETICULUM function. It triggers many responses, including UNFOLDED PROTEIN RESPONSE, which may lead to APOPTOSIS; and AUTOPHAGY. Stress, Endoplasmic Reticulum,Endoplasmic Reticulum Stresses,Reticulum Stress, Endoplasmic,Reticulum Stresses, Endoplasmic,Stresses, Endoplasmic Reticulum

Related Publications

Pengchen Chen, and Junhui Chen, and Wei Zhang, and Li Tang, and Guangqing Cheng, and Huiying Li, and Tianyun Fan, and Jigang Wang, and Wenbin Zhong, and Yali Song
December 2013, Toxicology,
Pengchen Chen, and Junhui Chen, and Wei Zhang, and Li Tang, and Guangqing Cheng, and Huiying Li, and Tianyun Fan, and Jigang Wang, and Wenbin Zhong, and Yali Song
December 2015, Environmental toxicology,
Pengchen Chen, and Junhui Chen, and Wei Zhang, and Li Tang, and Guangqing Cheng, and Huiying Li, and Tianyun Fan, and Jigang Wang, and Wenbin Zhong, and Yali Song
August 2006, Archives of pharmacal research,
Pengchen Chen, and Junhui Chen, and Wei Zhang, and Li Tang, and Guangqing Cheng, and Huiying Li, and Tianyun Fan, and Jigang Wang, and Wenbin Zhong, and Yali Song
January 2007, Toxicology,
Pengchen Chen, and Junhui Chen, and Wei Zhang, and Li Tang, and Guangqing Cheng, and Huiying Li, and Tianyun Fan, and Jigang Wang, and Wenbin Zhong, and Yali Song
February 1995, Toxicology,
Pengchen Chen, and Junhui Chen, and Wei Zhang, and Li Tang, and Guangqing Cheng, and Huiying Li, and Tianyun Fan, and Jigang Wang, and Wenbin Zhong, and Yali Song
December 2001, Toxicological sciences : an official journal of the Society of Toxicology,
Pengchen Chen, and Junhui Chen, and Wei Zhang, and Li Tang, and Guangqing Cheng, and Huiying Li, and Tianyun Fan, and Jigang Wang, and Wenbin Zhong, and Yali Song
May 2001, Journal of toxicology and environmental health. Part A,
Pengchen Chen, and Junhui Chen, and Wei Zhang, and Li Tang, and Guangqing Cheng, and Huiying Li, and Tianyun Fan, and Jigang Wang, and Wenbin Zhong, and Yali Song
March 2014, Environmental toxicology and pharmacology,
Pengchen Chen, and Junhui Chen, and Wei Zhang, and Li Tang, and Guangqing Cheng, and Huiying Li, and Tianyun Fan, and Jigang Wang, and Wenbin Zhong, and Yali Song
August 2015, Toxicology and applied pharmacology,
Pengchen Chen, and Junhui Chen, and Wei Zhang, and Li Tang, and Guangqing Cheng, and Huiying Li, and Tianyun Fan, and Jigang Wang, and Wenbin Zhong, and Yali Song
September 1989, Indian journal of experimental biology,
Copied contents to your clipboard!