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A 81-fold purification of human seminal plasma acid phosphatase was obtained by a three-step procedure, involving ammonium sulfate precipitation, DEAE-cellulose chromatography and Sephadex G-200 gel filtration. Homogeneity of the preparation during purification steps was tested by polyacrylamide gel electrophoresis and only one major band was obtained after the final step. The pH optimum for the activity of the purified enzyme was 5.6 and thermal stability was obtained even up to 40 degrees C. PNPP was the most specific synthetic substrate. The Km of purified seminal acid phosphatase towards PNPP was 1.5 X 10(-3) M. Among the metal ions tested, Hg+2 showed an I50 value of 4.2 X 10(-7) M. Studies with PCMB, PMSF and EDTA did not show any inhibition, whereas NaF and L(+)tartrate, at 1 mM concentration, inhibited the enzyme by 95% and 85%, respectively.
S K Nag Das, and
A Bhattacharyya, and
A K Bhattacharyya
January 1981,
Comptes rendus des seances de la Societe de biologie et de ses filiales,
S K Nag Das, and
A Bhattacharyya, and
A K Bhattacharyya
November 1988,
[Hokkaido igaku zasshi] The Hokkaido journal of medical science,
S K Nag Das, and
A Bhattacharyya, and
A K Bhattacharyya
January 1982,
Andrologia,
S K Nag Das, and
A Bhattacharyya, and
A K Bhattacharyya
December 1983,
The Biochemical journal,
S K Nag Das, and
A Bhattacharyya, and
A K Bhattacharyya
December 2008,
Molecular reproduction and development,
S K Nag Das, and
A Bhattacharyya, and
A K Bhattacharyya
March 2002,
Molecular human reproduction,
S K Nag Das, and
A Bhattacharyya, and
A K Bhattacharyya
January 1978,
Preparative biochemistry,
S K Nag Das, and
A Bhattacharyya, and
A K Bhattacharyya
January 1977,
Comptes rendus de l'Academie Bulgare des sciences : sciences mathematiques et naturelles,
S K Nag Das, and
A Bhattacharyya, and
A K Bhattacharyya
April 1974,
Folia endocrinologica,
S K Nag Das, and
A Bhattacharyya, and
A K Bhattacharyya
February 2003,
Theriogenology,
