Biomaterial Database

Immunochemical investigation of human seminal plasma acid phosphatase. 1982

G S Gupta, and D K Kapur

Acid phosphatase of human seminal plasma is inhibited in presence of normal rabbit serum involving non-competitive mechanism whereas this character is normalized and activity is stabilized in presence of its rabbit antiserum. Kinetic studies on stabilization of acid phosphatase by antiserum were combined by L-tartrate, a specific inhibitor of prostatic acid phosphatase. L-tartrate, a specific inhibitor of prostatic acid phosphatase. L-tartrate inhibited the enzyme in presence and absence of normal and antiserum by competitive mechanism and percentage of inhibition of L-tartrate was involve in the binding with acid phosphatase antibodies. The kinetics of heat denaturation of acid phosphatase suggested that seminal acid phosphatase is more heat labile in presence and absence of normal serum than in presence of antiserum. The temperature of denaturation corresponding to half the initial activity(T 1/2) in presence of normal and antisera were 34 degrees and 66 degrees C respectively. NOn-linearity of Arrhenius plots for denaturation rates of the enzyme suggested various conformational states for denaturation yielding Ea varying between 20.08 and 5.03 Kcals at 25-65 degrees C.

UI	MeSH Term	Description	Entries
D007158	Immunologic Techniques	Techniques used to demonstrate or measure an immune response, and to identify or measure antigens using antibodies.	Antibody Dissociation,Immunologic Technic,Immunologic Technics,Immunologic Technique,Immunological Technics,Immunological Techniques,Technic, Immunologic,Technics, Immunologic,Technique, Immunologic,Techniques, Immunologic,Antibody Dissociations,Dissociation, Antibody,Dissociations, Antibody,Immunological Technic,Immunological Technique,Technic, Immunological,Technics, Immunological,Technique, Immunological,Techniques, Immunological
D007700	Kinetics	The rate dynamics in chemical or physical systems.
D008297	Male		Males
D011467	Prostate	A gland in males that surrounds the neck of the URINARY BLADDER and the URETHRA. It secretes a substance that liquefies coagulated semen. It is situated in the pelvic cavity behind the lower part of the PUBIC SYMPHYSIS, above the deep layer of the triangular ligament, and rests upon the RECTUM.	Prostates
D011489	Protein Denaturation	Disruption of the non-covalent bonds and/or disulfide bonds responsible for maintaining the three-dimensional shape and activity of the native protein.	Denaturation, Protein,Denaturations, Protein,Protein Denaturations
D005260	Female		Females
D006358	Hot Temperature	Presence of warmth or heat or a temperature notably higher than an accustomed norm.	Heat,Hot Temperatures,Temperature, Hot,Temperatures, Hot
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000135	Acid Phosphatase	An enzyme that catalyzes the conversion of an orthophosphoric monoester and water to an alcohol and orthophosphate. EC 3.1.3.2.	Acid beta-Glycerophosphatase,Acid beta Glycerophosphatase
D001667	Binding, Competitive	The interaction of two or more substrates or ligands with the same binding site. The displacement of one by the other is used in quantitative and selective affinity measurements.	Competitive Binding