Biomaterial Database

Avian spermatozoa penetrate zona-free hamster oocytes in vitro. 1986

J Samour, and H D Moore, and C A Smith

After collection from the cloaca of adult males, budgerigar spermatozoa were incubated in modified BWW medium at pH 8.3. By light microscopy, many spermatozoa (greater than 100 per oocyte) were seen to be bound to the oolemma, and a small proportion (1-2 spermatozoa) were judged to have undergone fusion and penetration of the vitellus, as indicated by complete decondensation of their chromatin. The latter finding was confirmed at the electron microscope level by the presence of sperm nucleus, midpiece and tail in egg cytoplasm. This finding is discussed in relation to events at fertilization in the different Phyla.

UI	MeSH Term	Description	Entries
D008297	Male		Males
D008854	Microscopy, Electron	Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.	Electron Microscopy
D009865	Oocytes	Female germ cells derived from OOGONIA and termed OOCYTES when they enter MEIOSIS. The primary oocytes begin meiosis but are arrested at the diplotene state until OVULATION at PUBERTY to give rise to haploid secondary oocytes or ova (OVUM).	Ovocytes,Oocyte,Ovocyte
D010063	Ovum	A mature haploid female germ cell extruded from the OVARY at OVULATION.	Egg,Egg, Unfertilized,Ova,Eggs, Unfertilized,Unfertilized Egg,Unfertilized Eggs
D002171	Canaries	Any of several Old World finches of the genus Serinus.	Serinus,Canary
D005260	Female		Females
D006224	Cricetinae	A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.	Cricetus,Hamsters,Hamster
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D013084	Sperm-Ovum Interactions	Interactive processes between the oocyte (OVUM) and the sperm (SPERMATOZOA) including sperm adhesion, ACROSOME REACTION, sperm penetration of the ZONA PELLUCIDA, and events leading to FERTILIZATION.	Ovum-Sperm Interactions,Sperm Penetration,Egg-Sperm Interactions,Gamete Interactions,Oocyte-Sperm Interactions,Sperm-Egg Interactions,Sperm-Egg Penetration,Sperm-Oocyte Interactions,Sperm-Oocyte Penetration,Sperm-Ovum Penetration,Sperm-Zona Pellucida Penetration,Egg Sperm Interactions,Egg-Sperm Interaction,Gamete Interaction,Oocyte Sperm Interactions,Oocyte-Sperm Interaction,Ovum Sperm Interactions,Ovum-Sperm Interaction,Sperm Egg Interactions,Sperm Egg Penetration,Sperm Oocyte Interactions,Sperm Oocyte Penetration,Sperm Ovum Interactions,Sperm Ovum Penetration,Sperm Penetrations,Sperm Zona Pellucida Penetration,Sperm-Egg Interaction,Sperm-Egg Penetrations,Sperm-Oocyte Interaction,Sperm-Oocyte Penetrations,Sperm-Ovum Interaction,Sperm-Ovum Penetrations,Sperm-Zona Pellucida Penetrations
D013094	Spermatozoa	Mature male germ cells derived from SPERMATIDS. As spermatids move toward the lumen of the SEMINIFEROUS TUBULES, they undergo extensive structural changes including the loss of cytoplasm, condensation of CHROMATIN into the SPERM HEAD, formation of the ACROSOME cap, the SPERM MIDPIECE and the SPERM TAIL that provides motility.	Sperm,Spermatozoon,X-Bearing Sperm,X-Chromosome-Bearing Sperm,Y-Bearing Sperm,Y-Chromosome-Bearing Sperm,Sperm, X-Bearing,Sperm, X-Chromosome-Bearing,Sperm, Y-Bearing,Sperm, Y-Chromosome-Bearing,Sperms, X-Bearing,Sperms, X-Chromosome-Bearing,Sperms, Y-Bearing,Sperms, Y-Chromosome-Bearing,X Bearing Sperm,X Chromosome Bearing Sperm,X-Bearing Sperms,X-Chromosome-Bearing Sperms,Y Bearing Sperm,Y Chromosome Bearing Sperm,Y-Bearing Sperms,Y-Chromosome-Bearing Sperms