Biomaterial Database

Comparison of bovine sperm capacitation systems for ability of sperm to penetrate zona-free hamster oocytes and bovine oocytes matured in vitro. 1995

N Iqbal, and A G Hunter

Department of Animal Sciences, University of Minnesota, St. Paul 55108, USA.

Sperm from the same ejaculate were capacitated in calcium-free Tyrode's, modified Tyrode's with bovine follicular fluid or heparin, and TEST-yolk. Capacitated sperm were coincubated with zona-free hamster oocytes at 37 degrees C for 3 h or with bovine cumulus-enclosed oocytes matured in vitro for 8 h at 37 or 39 degrees C. Bovine oocytes were transferred to fertilization medium and incubated for 72 h at 37 or 39 degrees C. Penetration of zona-free hamster oocytes was highest in calcium-free Tyrode's (53.7%), followed by Tyrode's with follicular fluid (50.6%), TEST-yolk (48.6%), and Tyrode's with heparin (34.2%). Penetration of bovine cumulus-enclosed oocytes matured in vitro at 37 degrees C was highest with heparin (48.1%), followed by TEST-yolk (35.0%), calcium-free Tyrode's (32.5%), and follicular fluid (26.4%). Sperm capacitation with heparin, assayed by penetration of bovine oocytes at 39 degrees C, was better at 39 degrees C (77.1%) than at 37 degrees C (46.1%). However, penetration rates in calcium-free Tyrode's and TEST-yolk were higher at 37 degrees C. The capacitation system with heparin at 39 degrees C and bovine oocytes was best followed by the systems using TEST-yolk and calcium-free Tyrode's at 37 degrees C.

UI	MeSH Term	Description	Entries
D007552	Isotonic Solutions	Solutions having the same osmotic pressure as blood serum, or another solution with which they are compared. (From Grant & Hackh's Chemical Dictionary, 5th ed & Dorland, 28th ed)	Solutions, Isotonic
D008297	Male		Males
D002417	Cattle	Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.	Beef Cow,Bos grunniens,Bos indicus,Bos indicus Cattle,Bos taurus,Cow,Cow, Domestic,Dairy Cow,Holstein Cow,Indicine Cattle,Taurine Cattle,Taurus Cattle,Yak,Zebu,Beef Cows,Bos indicus Cattles,Cattle, Bos indicus,Cattle, Indicine,Cattle, Taurine,Cattle, Taurus,Cattles, Bos indicus,Cattles, Indicine,Cattles, Taurine,Cattles, Taurus,Cow, Beef,Cow, Dairy,Cow, Holstein,Cows,Dairy Cows,Domestic Cow,Domestic Cows,Indicine Cattles,Taurine Cattles,Taurus Cattles,Yaks,Zebus
D002478	Cells, Cultured	Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.	Cultured Cells,Cell, Cultured,Cultured Cell
D004338	Drug Combinations	Single preparations containing two or more active agents, for the purpose of their concurrent administration as a fixed dose mixture.	Drug Combination,Combination, Drug,Combinations, Drug
D005260	Female		Females
D006224	Cricetinae	A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.	Cricetus,Hamsters,Hamster
D006493	Heparin	A highly acidic mucopolysaccharide formed of equal parts of sulfated D-glucosamine and D-glucuronic acid with sulfaminic bridges. The molecular weight ranges from six to twenty thousand. Heparin occurs in and is obtained from liver, lung, mast cells, etc., of vertebrates. Its function is unknown, but it is used to prevent blood clotting in vivo and vitro, in the form of many different salts.	Heparinic Acid,alpha-Heparin,Heparin Sodium,Liquaemin,Sodium Heparin,Unfractionated Heparin,Heparin, Sodium,Heparin, Unfractionated,alpha Heparin
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D013075	Sperm Capacitation	The structural and functional changes by which SPERMATOZOA become capable of oocyte FERTILIZATION. It normally requires exposing the sperm to the female genital tract for a period of time to bring about increased SPERM MOTILITY and the ACROSOME REACTION before fertilization in the FALLOPIAN TUBES can take place.	Capacitation of Spermatozoa,Capacitation, Sperm,Spermatozoa Capacitation