Biomaterial Database

The influence of certain growth conditions on the phosphatase activity of Streptococcus mutans grown in batch and continuous culture. 1978

J Greenman, and T H Melville

Acid phosphatase activity was detected in Streptococcus mutans strain NCTC 10832, and both acid and alkaline phosphatase in strains 2M2 and K1R. In batch culture, activity was maximal by mid exponential phase for 2M2 and at the end of this phase for NCTC 10832. Alkaline, but not acid, phosphatase activity of 2M2 and K1R increased when the inorganic phosphate in the medium was low; this was considered due, at least partly, to inducible or derepressible enzymes. In continuous culture, acid phosphatase activity of NCTC 10832 varied with the sugar substrate. The activity was increased by cell disruption and the degree of this increase for cells grown on different sugars parallelled the amounts of extracellular, insoluble polysaccharide produced on those sugars. Activity was highest for glucose-grown whole cells and for sucrose-grown disrupted cells.

UI	MeSH Term	Description	Entries
D010710	Phosphates	Inorganic salts of phosphoric acid.	Inorganic Phosphate,Phosphates, Inorganic,Inorganic Phosphates,Orthophosphate,Phosphate,Phosphate, Inorganic
D002241	Carbohydrates	A class of organic compounds composed of carbon, hydrogen, and oxygen in a ratio of Cn(H2O)n. The largest class of organic compounds, including STARCH; GLYCOGEN; CELLULOSE; POLYSACCHARIDES; and simple MONOSACCHARIDES.	Carbohydrate
D003470	Culture Media	Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.	Media, Culture
D005947	Glucose	A primary source of energy for living organisms. It is naturally occurring and is found in fruits and other parts of plants in its free state. It is used therapeutically in fluid and nutrient replacement.	Dextrose,Anhydrous Dextrose,D-Glucose,Glucose Monohydrate,Glucose, (DL)-Isomer,Glucose, (alpha-D)-Isomer,Glucose, (beta-D)-Isomer,D Glucose,Dextrose, Anhydrous,Monohydrate, Glucose
D000135	Acid Phosphatase	An enzyme that catalyzes the conversion of an orthophosphoric monoester and water to an alcohol and orthophosphate. EC 3.1.3.2.	Acid beta-Glycerophosphatase,Acid beta Glycerophosphatase
D000469	Alkaline Phosphatase	An enzyme that catalyzes the conversion of an orthophosphoric monoester and water to an alcohol and orthophosphate. EC 3.1.3.1.
D001431	Bacteriological Techniques	Techniques used in studying bacteria.	Bacteriologic Technic,Bacteriologic Technics,Bacteriologic Techniques,Bacteriological Technique,Technic, Bacteriological,Technics, Bacteriological,Technique, Bacteriological,Techniques, Bacteriological,Bacteriologic Technique,Bacteriological Technic,Bacteriological Technics,Technic, Bacteriologic,Technics, Bacteriologic,Technique, Bacteriologic,Techniques, Bacteriologic
D013295	Streptococcus mutans	A polysaccharide-producing species of STREPTOCOCCUS isolated from human dental plaque.