BIOMAT Database Logo BIOMAT Database Logo

Genomics and Antimicrobial Susceptibility of Clinical Pseudomonas aeruginosa Isolates from Hospitals in Brazil. 2023

Carlos Henrique Camargo, and Amanda Yaeko Yamada, and Andreia Rodrigues de Souza, and Marisa de Jesus de Castro Lima, and Marcos Paulo Vieira Cunha, and Pedro Smith Pereira Ferraro, and Claudio Tavares Sacchi, and Marlon Benedito Nascimento Dos Santos, and Karoline Rodrigues Campos, and Monique Ribeiro Tiba-Casas, and Maristela Pinheiro Freire, and Pasqual Barretti
Centro de Bacteriologia, Instituto Adolfo Lutz, Sao Paulo 01246-902, SP, Brazil.

Pseudomonas aeruginosa, an opportunistic pathogen causing infections in immunocompromised patients, usually shows pronounced antimicrobial resistance. In recent years, the frequency of carbapenemases in P. aeruginosa has decreased, which allows use of new beta-lactams/combinations in antimicrobial therapy. Therefore, the in vitro evaluation of these drugs in contemporary isolates is warranted. We evaluated the antimicrobial susceptibility and genomic aspects of 119 clinical P. aeruginosa isolates from 24 different hospitals in Brazil in 2021-2022. Identification was performed via MALDI-TOF-MS, and antimicrobial susceptibility was identified through broth microdilution, gradient tests, or disk diffusion. Whole-genome sequencing was carried out using NextSeq equipment. The most active drug was cefiderocol (100%), followed by ceftazidime-avibactam (94.1%), ceftolozane-tazobactam (92.4%), and imipenem-relebactam (81.5%). Imipenem susceptibility was detected in 59 isolates (49.6%), and the most active aminoglycoside was tobramycin, to which 99 (83.2%) isolates were susceptible. Seventy-one different sequence types (STs) were detected, including twelve new STs described herein. The acquired resistance genes blaCTX-M-2 and blaKPC-2 were identified in ten (8.4%) and two (1.7%) isolates, respectively. Several virulence genes (exoSTUY, toxA, aprA, lasA/B, plcH) were also identified. We found that new antimicrobials are effective against the diverse P. aeruginosa population that has been circulating in Brazilian hospitals in recent years.

UI MeSH Term Description Entries

Related Publications

Carlos Henrique Camargo, and Amanda Yaeko Yamada, and Andreia Rodrigues de Souza, and Marisa de Jesus de Castro Lima, and Marcos Paulo Vieira Cunha, and Pedro Smith Pereira Ferraro, and Claudio Tavares Sacchi, and Marlon Benedito Nascimento Dos Santos, and Karoline Rodrigues Campos, and Monique Ribeiro Tiba-Casas, and Maristela Pinheiro Freire, and Pasqual Barretti
Comparison of the antimicrobial susceptibility patterns of Pseudomonas aeruginosa isolates from two hospitals.
December 1986, The Journal of the Oklahoma State Medical Association,
Carlos Henrique Camargo, and Amanda Yaeko Yamada, and Andreia Rodrigues de Souza, and Marisa de Jesus de Castro Lima, and Marcos Paulo Vieira Cunha, and Pedro Smith Pereira Ferraro, and Claudio Tavares Sacchi, and Marlon Benedito Nascimento Dos Santos, and Karoline Rodrigues Campos, and Monique Ribeiro Tiba-Casas, and Maristela Pinheiro Freire, and Pasqual Barretti
Antimicrobial drug susceptibility of clinical isolates of Pseudomonas aeruginosa.
March 1995, The Canadian veterinary journal = La revue veterinaire canadienne,
Carlos Henrique Camargo, and Amanda Yaeko Yamada, and Andreia Rodrigues de Souza, and Marisa de Jesus de Castro Lima, and Marcos Paulo Vieira Cunha, and Pedro Smith Pereira Ferraro, and Claudio Tavares Sacchi, and Marlon Benedito Nascimento Dos Santos, and Karoline Rodrigues Campos, and Monique Ribeiro Tiba-Casas, and Maristela Pinheiro Freire, and Pasqual Barretti
Antimicrobial susceptibility pattern of clinical isolates of Pseudomonas aeruginosa.
June 2004, Saudi medical journal,
Carlos Henrique Camargo, and Amanda Yaeko Yamada, and Andreia Rodrigues de Souza, and Marisa de Jesus de Castro Lima, and Marcos Paulo Vieira Cunha, and Pedro Smith Pereira Ferraro, and Claudio Tavares Sacchi, and Marlon Benedito Nascimento Dos Santos, and Karoline Rodrigues Campos, and Monique Ribeiro Tiba-Casas, and Maristela Pinheiro Freire, and Pasqual Barretti
Serotypes and antimicrobial susceptibility among clinical isolates of Pseudomonas aeruginosa.
May 1987, Gaoxiong yi xue ke xue za zhi = The Kaohsiung journal of medical sciences,
Carlos Henrique Camargo, and Amanda Yaeko Yamada, and Andreia Rodrigues de Souza, and Marisa de Jesus de Castro Lima, and Marcos Paulo Vieira Cunha, and Pedro Smith Pereira Ferraro, and Claudio Tavares Sacchi, and Marlon Benedito Nascimento Dos Santos, and Karoline Rodrigues Campos, and Monique Ribeiro Tiba-Casas, and Maristela Pinheiro Freire, and Pasqual Barretti
[Antimicrobial susceptibility of Pseudomonas aeruginosa clinical isolates in Murcia, Spain].
December 2003, Revista espanola de quimioterapia : publicacion oficial de la Sociedad Espanola de Quimioterapia,
Carlos Henrique Camargo, and Amanda Yaeko Yamada, and Andreia Rodrigues de Souza, and Marisa de Jesus de Castro Lima, and Marcos Paulo Vieira Cunha, and Pedro Smith Pereira Ferraro, and Claudio Tavares Sacchi, and Marlon Benedito Nascimento Dos Santos, and Karoline Rodrigues Campos, and Monique Ribeiro Tiba-Casas, and Maristela Pinheiro Freire, and Pasqual Barretti
[Antimicrobial susceptibility of Pseudomonas aeruginosa clinical isolates in Castellón, Spain].
March 2006, Revista espanola de quimioterapia : publicacion oficial de la Sociedad Espanola de Quimioterapia,
Carlos Henrique Camargo, and Amanda Yaeko Yamada, and Andreia Rodrigues de Souza, and Marisa de Jesus de Castro Lima, and Marcos Paulo Vieira Cunha, and Pedro Smith Pereira Ferraro, and Claudio Tavares Sacchi, and Marlon Benedito Nascimento Dos Santos, and Karoline Rodrigues Campos, and Monique Ribeiro Tiba-Casas, and Maristela Pinheiro Freire, and Pasqual Barretti
Antimicrobial susceptibility of clinical isolates of Pseudomonas aeruginosa from a Malaysian Hospital.
April 2009, The Malaysian journal of medical sciences : MJMS,
Carlos Henrique Camargo, and Amanda Yaeko Yamada, and Andreia Rodrigues de Souza, and Marisa de Jesus de Castro Lima, and Marcos Paulo Vieira Cunha, and Pedro Smith Pereira Ferraro, and Claudio Tavares Sacchi, and Marlon Benedito Nascimento Dos Santos, and Karoline Rodrigues Campos, and Monique Ribeiro Tiba-Casas, and Maristela Pinheiro Freire, and Pasqual Barretti
Antimicrobial susceptibility of Pseudomonas aeruginosa isolates obtained from patients in Canadian hospitals: CANWARD 2008-2011.
August 2012, Diagnostic microbiology and infectious disease,
Carlos Henrique Camargo, and Amanda Yaeko Yamada, and Andreia Rodrigues de Souza, and Marisa de Jesus de Castro Lima, and Marcos Paulo Vieira Cunha, and Pedro Smith Pereira Ferraro, and Claudio Tavares Sacchi, and Marlon Benedito Nascimento Dos Santos, and Karoline Rodrigues Campos, and Monique Ribeiro Tiba-Casas, and Maristela Pinheiro Freire, and Pasqual Barretti
Antimicrobial susceptibility profile of Pseudomonas aeruginosa isolates in Egypt.
July 2008, The Journal of urology,
Carlos Henrique Camargo, and Amanda Yaeko Yamada, and Andreia Rodrigues de Souza, and Marisa de Jesus de Castro Lima, and Marcos Paulo Vieira Cunha, and Pedro Smith Pereira Ferraro, and Claudio Tavares Sacchi, and Marlon Benedito Nascimento Dos Santos, and Karoline Rodrigues Campos, and Monique Ribeiro Tiba-Casas, and Maristela Pinheiro Freire, and Pasqual Barretti
Antimicrobial susceptibility of Pseudomonas aeruginosa isolates from dogs with otitis externa.
July 2011, The Veterinary record,
Copied contents to your clipboard!