Biomaterial Database

Fabrication of 3D Printed Hollow Microneedles by Digital Light Processing for the Buccal Delivery of Actives. 2023

Paraskevi Kyriaki Monou, and Eleftherios G Andriotis, and Konstantinos Tsongas, and Emmanouil K Tzimtzimis, and Orestis L Katsamenis, and Dimitrios Tzetzis, and Pinelopi Anastasiadou, and Christos Ritzoulis, and Ioannis S Vizirianakis, and Dimitrios Andreadis, and Dimitrios G Fatouros

Department of Pharmacy Division of Pharmaceutical Technology, Aristotle University of Thessaloniki, Thessaloniki 54124, Greece.

In the present study, two different microneedle devices were produced using digital light processing (DLP). These devices hold promise as drug delivery systems to the buccal tissue as they increase the permeability of actives with molecular weights between 600 and 4000 Da. The attached reservoirs were designed and printed along with the arrays as a whole device. Light microscopy was used to quality control the printability of the designs, confirming that the actual dimensions are in agreement with the digital design. Non-destructive volume imaging by means of microfocus computed tomography was employed for dimensional and defect characterization of the DLP-printed devices, demonstrating the actual volumes of the reservoirs and the malformations that occurred during printing. The penetration test and finite element analysis showed that the maximum stress experienced by the needles during the insertion process (10 N) was below their ultimate compressive strength (240-310 N). Permeation studies showed the increased permeability of three model drugs when delivered with the MN devices. Size-exclusion chromatography validated the stability of all the actives throughout the permeability tests. The safety of these printed devices for buccal administration was confirmed by histological evaluation and cell viability studies using the TR146 cell line, which indicated no toxic effects.

UI	MeSH Term	Description	Entries
D008027	Light	That portion of the electromagnetic spectrum in the visible, ultraviolet, and infrared range.	Light, Visible,Photoradiation,Radiation, Visible,Visible Radiation,Photoradiations,Radiations, Visible,Visible Light,Visible Radiations
D009339	Needles	Sharp instruments used for puncturing or suturing.	Hypodermic Needles,Hypodermic Needle,Needle,Needle, Hypodermic,Needles, Hypodermic
D002460	Cell Line	Established cell cultures that have the potential to propagate indefinitely.	Cell Lines,Line, Cell,Lines, Cell
D002470	Cell Survival	The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability.	Cell Viability,Cell Viabilities,Survival, Cell,Viabilities, Cell,Viability, Cell
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D066330	Printing, Three-Dimensional	Process for making, building or constructing a physical object from a three-dimensional digital model by laying down many successive thin layers of building material.	3-D Printing,3-Dimensional Printing,3D Printing,Three-Dimensional Printing,3 D Printing,3 Dimensional Printing,3-D Printings,3-Dimensional Printings,3D Printings,Printing, 3-D,Printing, 3-Dimensional,Printing, 3D,Printing, Three Dimensional,Printings, 3-D,Printings, 3-Dimensional,Printings, 3D,Printings, Three-Dimensional,Three Dimensional Printing,Three-Dimensional Printings