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The effect of phorbol esters on calcium-activated, phospholipid-dependent kinase (protein kinase C) and luteinizing hormone (LH) secretion was examined in cultured rat anterior pituitary cells. The potent tumor promoter 12-O-tetra-decanoylphorbol-13-acetate (TPA) stimulated LH secretion and activated pituitary protein kinase C in the presence of calcium and phosphatidylserine. The enzyme activity present in cytosol and particulate fractions was eluted at about 0.05 M NaCl during DE52-cellulose chromatography. Preincubation of pituitary cells with TPA markedly decreased cytosolic protein kinase C activity and increased enzyme activity in the particulate fraction. The maximal TPA-induced change in enzyme activity, with a 76% decrease in cytosol and a 4.3-fold increase in the particulate fraction, occurred within 10 min. The dose-dependent changes in protein kinase C redistribution in TPA-treated cells were correlated with the stimulation of LH release by the phorbol ester. These results suggest that activation of protein kinase C by TPA is associated with intracellular redistribution of the enzyme and is related to the process of secretory granule release from gonadotrophs.
K Hirota, and
T Hirota, and
G Aguilera, and
K J Catt
December 1985,
Proceedings of the National Academy of Sciences of the United States of America,
K Hirota, and
T Hirota, and
G Aguilera, and
K J Catt
November 1985,
FEBS letters,
K Hirota, and
T Hirota, and
G Aguilera, and
K J Catt
April 1986,
Carcinogenesis,
K Hirota, and
T Hirota, and
G Aguilera, and
K J Catt
October 1986,
Molecular and cellular endocrinology,
K Hirota, and
T Hirota, and
G Aguilera, and
K J Catt
January 1983,
Methods in enzymology,
K Hirota, and
T Hirota, and
G Aguilera, and
K J Catt
June 1985,
The American journal of physiology,
K Hirota, and
T Hirota, and
G Aguilera, and
K J Catt
July 1982,
The Journal of biological chemistry,
K Hirota, and
T Hirota, and
G Aguilera, and
K J Catt
February 1986,
Biochemical and biophysical research communications,
K Hirota, and
T Hirota, and
G Aguilera, and
K J Catt
February 1987,
Biochemical and biophysical research communications,
K Hirota, and
T Hirota, and
G Aguilera, and
K J Catt
December 1986,
Cellular immunology,
