-transparent.png){kind=logo}
We describe a fluorescence polarization assay for use in predicting fetal lung maturity, which is suitable for the TDx Analyzer (an automated fluorescence polarimeter). The assay requires 0.5 mL of amniotic fluid and approximately 30 min, and involves the fluorophore 1-palmitoyl-2(6-[(7-nitro-2, 1, 3-benzoxadiazol-4-yl)amino]caproyl) phosphatidylcholine. Reproducible polarization measurements depend on proper regulation of incubation time and temperature, but variations in the concentrations of fluorescent probe and amniotic fluid have little effect on measured polarization and therefore little effect on assay precision. Working solutions of the fluorescent probe are stable for at least nine months when stored at -20 degrees C and pH 5. Interferences include erythrocytes, serum, bilirubin, meconium, and lidocaine.
J F Tait, and
R W Franklin, and
J B Simpson, and
E R Ashwood
April 1987,
Clinical chemistry,
J F Tait, and
R W Franklin, and
J B Simpson, and
E R Ashwood
July 1987,
Clinical chemistry,
J F Tait, and
R W Franklin, and
J B Simpson, and
E R Ashwood
October 1992,
Obstetrics and gynecology,
J F Tait, and
R W Franklin, and
J B Simpson, and
E R Ashwood
March 1982,
Clinical chemistry,
J F Tait, and
R W Franklin, and
J B Simpson, and
E R Ashwood
April 1986,
Biochemical pharmacology,
J F Tait, and
R W Franklin, and
J B Simpson, and
E R Ashwood
January 1993,
Journal of perinatal medicine,
J F Tait, and
R W Franklin, and
J B Simpson, and
E R Ashwood
August 1983,
Clinical chemistry,
J F Tait, and
R W Franklin, and
J B Simpson, and
E R Ashwood
October 1995,
Southern medical journal,
J F Tait, and
R W Franklin, and
J B Simpson, and
E R Ashwood
April 1978,
American journal of obstetrics and gynecology,
J F Tait, and
R W Franklin, and
J B Simpson, and
E R Ashwood
March 1986,
Clinical chemistry,
