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Rat apoA-IV complexes with dimyristoyl phosphatidylcholine (apoA-IV-DMPC) have been prepared and their ability to bind to purified rat liver plasma membranes investigated. Binding equilibrium at 37 degrees C was reached in 30 minutes. Saturation binding experiments and subsequent analysis of the results with Scatchard plots gave results consistent with the presence of a single saturable binding site. DMPC or POPC unilamellar vesicles could not compete with apoA-IV-DMPC for binding; apoA-I-DMPC competed only partially. ApoE-poor HDL effectively competed with apoA-IV-DMPC. The fact that binding could be greatly reduced (greater than 70%) by preincubating the membrane with pronase (18 micrograms/ml), supports the conclusion that a membrane protein is involved in binding. Based on these results, we speculate that the rapid catabolism of apoA-IV in plasma may be mediated by a specific uptake mechanism in the liver. The implications of these results support the hypothesis that apoA-IV is involved in reverse cholesterol transport.
G Ghiselli, and
W L Crump, and
A M Gotto
April 1983,
Biochemical and biophysical research communications,
G Ghiselli, and
W L Crump, and
A M Gotto
June 1984,
Endocrinologia japonica,
G Ghiselli, and
W L Crump, and
A M Gotto
May 1977,
Hoppe-Seyler's Zeitschrift fur physiologische Chemie,
G Ghiselli, and
W L Crump, and
A M Gotto
February 1986,
Experimental cell research,
G Ghiselli, and
W L Crump, and
A M Gotto
January 1991,
Journal of lipid mediators,
G Ghiselli, and
W L Crump, and
A M Gotto
September 1971,
FEBS letters,
G Ghiselli, and
W L Crump, and
A M Gotto
December 1981,
Agents and actions,
G Ghiselli, and
W L Crump, and
A M Gotto
July 1977,
Biochemical and biophysical research communications,
G Ghiselli, and
W L Crump, and
A M Gotto
January 1986,
Biochimie,
G Ghiselli, and
W L Crump, and
A M Gotto
May 1979,
Biochemical and biophysical research communications,
