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Antisera raised in rabbits against synthetic insulin-like growth factor-II (IGF-II) were used to develop a specific radioimmunoassay (RIA) for IGF-II. Affinity purified antibodies showed 6% cross-reactivity with IGF-I but failed to recognize insulin even at 10 micrograms/tube. Utilizing this RIA system, immunoreactive IGF-II was identified in the pooled samples of human follicular fluid and seminal plasma. The acid-ethanol precipitates of human seminal and follicular fluids were chromatographed on Sephadex G-50 column and the IGF-II immunoreactive fractions were subjected to reversed-phase high performance liquid chromatography. It was found that immunoactive IGF-II was eluted in the same location as that of synthetic IGF-II. The data indicate for the first time that human seminal plasma and follicular fluid contain significant amounts of IGF-II.
K Ramasharma, and
C M Cabrera, and
C H Li
January 1986,
Biochemical and biophysical research communications,
K Ramasharma, and
C M Cabrera, and
C H Li
January 1989,
Human reproduction (Oxford, England),
K Ramasharma, and
C M Cabrera, and
C H Li
October 1985,
Proceedings of the National Academy of Sciences of the United States of America,
K Ramasharma, and
C M Cabrera, and
C H Li
February 1994,
The Journal of biological chemistry,
K Ramasharma, and
C M Cabrera, and
C H Li
January 1990,
Acta paediatrica Scandinavica. Supplement,
K Ramasharma, and
C M Cabrera, and
C H Li
November 1990,
The Journal of clinical endocrinology and metabolism,
K Ramasharma, and
C M Cabrera, and
C H Li
May 1991,
Hepatology (Baltimore, Md.),
K Ramasharma, and
C M Cabrera, and
C H Li
May 1978,
FEBS letters,
K Ramasharma, and
C M Cabrera, and
C H Li
January 1994,
Fertility and sterility,
K Ramasharma, and
C M Cabrera, and
C H Li
December 1991,
The Biochemical journal,
