BIOMAT Database Logo BIOMAT Database Logo

Electron microscopy microsampling of isolated nuclei sorted by flow cytometry. 1986

N Zini, and A Matteucci, and S Squarzoni, and A Galanzi, and R Rizzoli, and S Papa

This paper describes an efficient method to concentrate for electron microscopic examination minute quantities of subcellular particles obtained by cytofluorimetric sorting. The advantages of this micromethod, based on diafiltration on Millipore filters under constant positive nitrogen pressure, are discussed.

UI MeSH Term Description Entries
D008099 Liver A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances. Livers
D008856 Microscopy, Fluorescence Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. Immunofluorescence microscopy utilizes antibodies that are labeled with fluorescent dye. Fluorescence Microscopy,Immunofluorescence Microscopy,Microscopy, Immunofluorescence,Fluorescence Microscopies,Immunofluorescence Microscopies,Microscopies, Fluorescence,Microscopies, Immunofluorescence
D002467 Cell Nucleus Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed) Cell Nuclei,Nuclei, Cell,Nucleus, Cell
D002469 Cell Separation Techniques for separating distinct populations of cells. Cell Isolation,Cell Segregation,Isolation, Cell,Cell Isolations,Cell Segregations,Cell Separations,Isolations, Cell,Segregation, Cell,Segregations, Cell,Separation, Cell,Separations, Cell
D005434 Flow Cytometry Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake. Cytofluorometry, Flow,Cytometry, Flow,Flow Microfluorimetry,Fluorescence-Activated Cell Sorting,Microfluorometry, Flow,Cell Sorting, Fluorescence-Activated,Cell Sortings, Fluorescence-Activated,Cytofluorometries, Flow,Cytometries, Flow,Flow Cytofluorometries,Flow Cytofluorometry,Flow Cytometries,Flow Microfluorometries,Flow Microfluorometry,Fluorescence Activated Cell Sorting,Fluorescence-Activated Cell Sortings,Microfluorimetry, Flow,Microfluorometries, Flow,Sorting, Fluorescence-Activated Cell,Sortings, Fluorescence-Activated Cell
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D013048 Specimen Handling Procedures for collecting, preserving, and transporting of specimens sufficiently stable to provide accurate and precise results suitable for clinical interpretation. Specimen Collection,Collection, Specimen,Collections, Specimen,Handling, Specimen,Handlings, Specimen,Specimen Collections,Specimen Handlings
D051381 Rats The common name for the genus Rattus. Rattus,Rats, Laboratory,Rats, Norway,Rattus norvegicus,Laboratory Rat,Laboratory Rats,Norway Rat,Norway Rats,Rat,Rat, Laboratory,Rat, Norway,norvegicus, Rattus

Related Publications

N Zini, and A Matteucci, and S Squarzoni, and A Galanzi, and R Rizzoli, and S Papa
Modifications of the chromatin arrangement induced by ethidium bromide in isolated nuclei, analyzed by electron microscopy and flow cytometry.
January 1987, Biology of the cell,
N Zini, and A Matteucci, and S Squarzoni, and A Galanzi, and R Rizzoli, and S Papa
Hyperexpansion of wheat chromosomes sorted by flow cytometry.
January 2014, Genes & genetic systems,
N Zini, and A Matteucci, and S Squarzoni, and A Galanzi, and R Rizzoli, and S Papa
PCR amplification of chromosome-specific DNA isolated from flow cytometry-sorted chromosomes.
February 1992, Genomics,
N Zini, and A Matteucci, and S Squarzoni, and A Galanzi, and R Rizzoli, and S Papa
Carbon-isotopic analysis of microbial cells sorted by flow cytometry.
March 2007, Geobiology,
N Zini, and A Matteucci, and S Squarzoni, and A Galanzi, and R Rizzoli, and S Papa
RNA and DNA content of isolated cell nuclei measured by multiparameter flow cytometry.
January 1983, Cytometry,
N Zini, and A Matteucci, and S Squarzoni, and A Galanzi, and R Rizzoli, and S Papa
Electron microscopy of cell nuclei isolated in aqueous media.
August 1956, Experimental cell research,
N Zini, and A Matteucci, and S Squarzoni, and A Galanzi, and R Rizzoli, and S Papa
DNA identification of sperm cells collected and sorted by flow cytometry.
September 2003, The American journal of forensic medicine and pathology,
N Zini, and A Matteucci, and S Squarzoni, and A Galanzi, and R Rizzoli, and S Papa
Isolation of full-size mRNA from cells sorted by flow cytometry.
August 1999, Journal of biochemical and biophysical methods,
N Zini, and A Matteucci, and S Squarzoni, and A Galanzi, and R Rizzoli, and S Papa
Gene expression in rod shaped cardiac myocytes, sorted by flow cytometry.
December 1998, Cardiovascular research,
N Zini, and A Matteucci, and S Squarzoni, and A Galanzi, and R Rizzoli, and S Papa
Flow cytometry of isolated nuclei prepared from 9L rat brain tumor.
July 1979, Laboratory investigation; a journal of technical methods and pathology,
Copied contents to your clipboard!