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Metabolic and chromosomal changes in a Bacillus subtilis whiA mutant. 2023
Laura C Bohorquez, and
Joana de Sousa, and
Transito Garcia-Garcia, and
Gaurav Dugar, and
Biwen Wang, and
Martijs J Jonker, and
Marie-Françoise Noirot-Gros, and
Michael Lalk, and
Leendert W Hamoen
Swammerdam Institute for Life Sciences, University of Amsterdam , Amsterdam, the Netherlands.
WhiA is a conserved DNA-binding protein that influences cell division in many Gram-positive bacteria and, in B. subtilis, also chromosome segregation. How WhiA works in Bacillus subtilis is unknown. Here, we tested three hypothetical mechanisms using metabolomics, fatty acid analysis, and chromosome confirmation capture experiments. This revealed that WhiA does not influence cell division and chromosome segregation by modulating either central carbon metabolism or fatty acid composition. However, the inactivation of WhiA reduces short-range chromosome interactions. These findings provide new avenues to study the molecular mechanism of WhiA in the future.
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