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A system was developed to permit perfusion of local brain regions and simultaneous peripheral blood sampling in free-moving caged monkeys. The system comprises a calvarial headpiece that contains multiple push-pull cannulas (PPC), a flexible stainless steel tether, a four-channel fluid swivel, and a surgical procedure for simultaneous multisite brain cannulation. Rhesus macaques were fitted surgically with an indwelling jugular catheter and PPC directed into the third ventricle, median eminence, and preoptic area. These animals were tethered for periods of 14-70 h during which brain perfusates and peripheral blood samples were collected at 10- to 30-min intervals through the tether-swivel assembly. Levels and pulsatile patterns of gonadotropin-releasing hormone in 10-min perfusate samples and luteinizing hormone and cortisol in sequential plasma samples were quantified by specific radioimmunoassays. The normal endocrine profiles in these animals suggest that this system provides a valuable method to study patterns of neurosecretions in an unrestrained simian.
P M Gliessman, and
K Y Pau, and
J D Hill, and
H G Spies
January 1986,
Annals of the New York Academy of Sciences,
P M Gliessman, and
K Y Pau, and
J D Hill, and
H G Spies
January 1987,
Annales d'endocrinologie,
P M Gliessman, and
K Y Pau, and
J D Hill, and
H G Spies
January 1979,
Neuroscience,
P M Gliessman, and
K Y Pau, and
J D Hill, and
H G Spies
January 1986,
Pharmacology, biochemistry, and behavior,
P M Gliessman, and
K Y Pau, and
J D Hill, and
H G Spies
January 1986,
Annals of the New York Academy of Sciences,
P M Gliessman, and
K Y Pau, and
J D Hill, and
H G Spies
January 1979,
The American journal of physiology,
P M Gliessman, and
K Y Pau, and
J D Hill, and
H G Spies
November 2002,
Journal of neuroscience methods,
P M Gliessman, and
K Y Pau, and
J D Hill, and
H G Spies
April 1977,
Pharmacology, biochemistry, and behavior,
P M Gliessman, and
K Y Pau, and
J D Hill, and
H G Spies
November 1983,
Electroencephalography and clinical neurophysiology,
P M Gliessman, and
K Y Pau, and
J D Hill, and
H G Spies
July 1988,
Brain research bulletin,
