The cytotoxicity of one conventional and four non-gamma 2-amalgams was studied in a cell culture system, using the Millipore filter method. Before testing set amalgam specimens were kept in distilled water or in artificial saliva at pH 4, 5, or 7 for up to 28 wk to produce a corrosion layer on the test surface. Non-corroded set amalgam specimens was also tested. None of the noncorroded, set amalgams showed any sign of surface accumulation of cytotoxic products whereas the corroded amalgams showed varying degrees of cytotoxicity. Generally, the non-gamma 2-amalgams gave a more pronounced cytotoxic effect than the conventional amalgam. When the corrosion procedure was carried out at pH 7, the various non-gamma 2-amalgams showed different degrees of cytotoxicity. It appears that the difference in cytotoxic effect between the non-gamma 2-amalgams and the conventional amalgam as well as the differences among the various non-gamma 2-amalgams could be related to variation in the retention of corrosion products deposited on the amalgam surface.