-transparent.png){kind=logo}
The DC and DX subregions of the human major histocompatibility complex (MHC) have been cloned from a cosmid library made from a human B-cell line, Priess. The DC subregion, 48 kilobases, includes the DC alpha and DC beta genes. A second DC-like region, the DX subregion, 35 kilobases, contains the DX alpha gene and a newly found beta gene termed DX beta. Since the DC and DX genes are highly homologous in nucleotide sequence, gene size, exon-intron organization, and direction of transcription, the DC and DX subregions were presumably generated by duplication of an ancestral alpha-beta gene pair. Nucleotide sequencing indicates that all four genes have intact coding sequences and promoter regions. Homology between the upstream promoter sequences of these four genes and seven other class II genes at nucleotides -69 to -78 and -98 to -110 highlights these previously described conserved elements. Moreover, a striking conservation of flanking alpha-gene-specific and beta-gene-specific sequences has been observed. Comparison of Southern blots of Priess DNA with DC alpha and DC beta cDNA probes with isolated cosmid clones showed that (i) the human chromosome encodes only two DC alpha-related and two DC beta-related genes, namely, DC alpha, DX alpha, DC beta, and DX beta, and (ii) the DC and DX subregions are homozygous in Priess cells.
K Okada, and
J M Boss, and
H Prentice, and
T Spies, and
R Mengler, and
C Auffray, and
J Lillie, and
D Grossberger, and
J L Strominger
May 1987,
Poultry science,
K Okada, and
J M Boss, and
H Prentice, and
T Spies, and
R Mengler, and
C Auffray, and
J Lillie, and
D Grossberger, and
J L Strominger
January 1986,
Lijecnicki vjesnik,
K Okada, and
J M Boss, and
H Prentice, and
T Spies, and
R Mengler, and
C Auffray, and
J Lillie, and
D Grossberger, and
J L Strominger
August 1984,
Proceedings of the National Academy of Sciences of the United States of America,
K Okada, and
J M Boss, and
H Prentice, and
T Spies, and
R Mengler, and
C Auffray, and
J Lillie, and
D Grossberger, and
J L Strominger
February 1989,
The Journal of experimental medicine,
K Okada, and
J M Boss, and
H Prentice, and
T Spies, and
R Mengler, and
C Auffray, and
J Lillie, and
D Grossberger, and
J L Strominger
December 1983,
Proceedings of the National Academy of Sciences of the United States of America,
K Okada, and
J M Boss, and
H Prentice, and
T Spies, and
R Mengler, and
C Auffray, and
J Lillie, and
D Grossberger, and
J L Strominger
March 1986,
Cell,
K Okada, and
J M Boss, and
H Prentice, and
T Spies, and
R Mengler, and
C Auffray, and
J Lillie, and
D Grossberger, and
J L Strominger
January 1987,
Immunologic research,
K Okada, and
J M Boss, and
H Prentice, and
T Spies, and
R Mengler, and
C Auffray, and
J Lillie, and
D Grossberger, and
J L Strominger
May 1990,
Genetics,
K Okada, and
J M Boss, and
H Prentice, and
T Spies, and
R Mengler, and
C Auffray, and
J Lillie, and
D Grossberger, and
J L Strominger
October 1983,
Journal of immunogenetics,
K Okada, and
J M Boss, and
H Prentice, and
T Spies, and
R Mengler, and
C Auffray, and
J Lillie, and
D Grossberger, and
J L Strominger
October 1992,
Pathologie-biologie,
