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Human T cell lines, MT-2, TCL-Ter, TCL-Haz, and TCL-Kan which were transformed by a human retrovirus, constitutively produced cytotoxic factor(s) (CF) in the culture supernatants. In these cell lines, MT-2 produced the largest amount of CF. The amount of CF produced by MT-2 was 9-10 or 3-4 times larger than that produced by a human B cell line, RPMI 1788, or normal peripheral blood leukocytes stimulated with mitogens and phorbol ester. The kinetics of the production by MT-2 was similar in media with and without serum. The activity was stable at 56 degrees C for 30 min but was lost at 80 degrees C for 30 min and at pH 2 for 20 hr. On gel filtration, the molecular weight of the factor produced by MT-2 was approximately 90,000. On isoelectric focusing, the activity was recovered in the fraction at pH 6.5-7.0.
S Hinuma, and
K Naruo, and
K Tsukamoto, and
K Sugamura, and
Y Hinuma
January 1984,
Microbiology and immunology,
S Hinuma, and
K Naruo, and
K Tsukamoto, and
K Sugamura, and
Y Hinuma
May 1981,
Nature,
S Hinuma, and
K Naruo, and
K Tsukamoto, and
K Sugamura, and
Y Hinuma
June 1985,
British journal of cancer,
S Hinuma, and
K Naruo, and
K Tsukamoto, and
K Sugamura, and
Y Hinuma
January 1984,
Microbiology and immunology,
S Hinuma, and
K Naruo, and
K Tsukamoto, and
K Sugamura, and
Y Hinuma
October 1994,
Blood,
S Hinuma, and
K Naruo, and
K Tsukamoto, and
K Sugamura, and
Y Hinuma
January 1985,
Microbiology and immunology,
S Hinuma, and
K Naruo, and
K Tsukamoto, and
K Sugamura, and
Y Hinuma
April 1982,
Journal of immunology (Baltimore, Md. : 1950),
S Hinuma, and
K Naruo, and
K Tsukamoto, and
K Sugamura, and
Y Hinuma
August 1981,
International journal of cancer,
S Hinuma, and
K Naruo, and
K Tsukamoto, and
K Sugamura, and
Y Hinuma
October 1984,
Gan,
S Hinuma, and
K Naruo, and
K Tsukamoto, and
K Sugamura, and
Y Hinuma
January 1981,
Microbiology and immunology,
