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The importance of the base composition and of the conformation of nucleic acids in the reaction with the drug cis-diamminedichloroplatinum(II) has been studied by competition experiments between the drug and several double-stranded polydeoxyribonucleotides. Binding to poly(dG).poly(dC) is larger than to poly (dG-dC).poly(dG-dC). There is no preferential binding in the competition between poly(dG-dC).poly(dG-dC), poly(dA-dC).poly(dG-dT) and poly(dA-dG).poly(dC-dT). In the competition between poly(dG-dC).poly (dG-dC) (B conformation) and poly(dG-br5dC).poly(dG-br5dC) (Z conformation), the drug binds equally well to both polynucleotides. In natural DNA, modification of guanine residues in (GC)n.(GC)n sequences by the drug has been revealed by the inhibition of cleavage of these sequences by the restriction enzyme BssHII. By means of antibodies to platinated poly(dG-dC), it is shown that some of the adducts formed in platinated poly(dG-dC) are also formed in platinated pBR322 DNA. The type of adducts recognized the antibodies is not known. Thin layer chromatography of the products after chemical and enzymatic hydrolysis of platinated poly(dG-dC) suggests that interstrand cross-links are formed. Finally, the conformations of poly(dG-dC) modified either by cis-diamminedichloroplatinum(II) or by trans-diamminedichloroplatinum (II) have been compared by circular dichroism. Both the cis-isomer and the trans-isomer stabilize the Z conformation when they bind to poly(dG-m5dC) in the Z conformation. When they bind to poly(dG-m5dC) in the B conformation, the conformations of poly(dG-m5dC) modified by the cis or the trans-isomer are different. Moreover, the cis-isomer facilitates the B form-Z form transition of the unplatinated regions while the trans-isomer makes it more difficult.
A Rahmouni, and
J M Malinge, and
A Schwartz, and
M Leng
July 1984,
Nucleic acids research,
A Rahmouni, and
J M Malinge, and
A Schwartz, and
M Leng
November 1984,
Nucleic acids research,
A Rahmouni, and
J M Malinge, and
A Schwartz, and
M Leng
March 1989,
Biopolymers,
A Rahmouni, and
J M Malinge, and
A Schwartz, and
M Leng
August 1981,
Proceedings of the National Academy of Sciences of the United States of America,
A Rahmouni, and
J M Malinge, and
A Schwartz, and
M Leng
November 1981,
Nucleic acids research,
A Rahmouni, and
J M Malinge, and
A Schwartz, and
M Leng
January 1982,
Proceedings of the National Academy of Sciences of the United States of America,
A Rahmouni, and
J M Malinge, and
A Schwartz, and
M Leng
March 1981,
Nucleic acids research,
A Rahmouni, and
J M Malinge, and
A Schwartz, and
M Leng
February 1988,
Biochimie,
A Rahmouni, and
J M Malinge, and
A Schwartz, and
M Leng
January 1984,
The Journal of biological chemistry,
A Rahmouni, and
J M Malinge, and
A Schwartz, and
M Leng
October 1983,
FEBS letters,
