Biomaterial Database

Detection of repair of chemical-induced DNA damage in vivo by the nucleoid sedimentation assay. 1985

F Romagna, and M S Kulkarni, and M W Anderson

The nucleoid sedimentation assay was used to study chemical-induced DNA repair in vivo. Nucleoid bodies were prepared from liver and lung of mice at various times after i.p. treatment with 1-methyl-1-nitrosourea or 4-nitroquinoline-1-oxide. Both carcinogens induced a dose-dependent loss in negative DNA supercoiling in liver and lung. The rate of DNA repair of 1-methyl-1-nitrosourea was similar in liver and lung whereas 4-nitroquinoline-1-oxide-induced DNA damage was repaired faster in lung than in liver. Results obtained by the nucleoid sedimentation technique corresponded to measurements of DNA repair by unscheduled DNA synthesis. The nucleoid sedimentation assay should be a useful tool to examine in vivo repair of chemical-induced DNA lesions in various tissues of laboratory animals.

UI	MeSH Term	Description	Entries
D008099	Liver	A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.	Livers
D008168	Lung	Either of the pair of organs occupying the cavity of the thorax that effect the aeration of the blood.	Lungs
D008297	Male		Males
D008722	Methods	A series of steps taken in order to conduct research.	Techniques,Methodological Studies,Methodological Study,Procedures,Studies, Methodological,Study, Methodological,Method,Procedure,Technique
D008770	Methylnitrosourea	A nitrosourea compound with alkylating, carcinogenic, and mutagenic properties.	Nitrosomethylurea,N-Methyl-N-nitrosourea,NSC-23909,N Methyl N nitrosourea,NSC 23909,NSC23909
D009600	Nitroquinolines	Quinolines substituted in any position by one or more nitro groups.
D009607	Nitrosourea Compounds	A class of compounds in which the core molecule is R-NO, where R is UREA.	Compounds, Nitrosourea
D009690	Nucleic Acid Conformation	The spatial arrangement of the atoms of a nucleic acid or polynucleotide that results in its characteristic 3-dimensional shape.	DNA Conformation,RNA Conformation,Conformation, DNA,Conformation, Nucleic Acid,Conformation, RNA,Conformations, DNA,Conformations, Nucleic Acid,Conformations, RNA,DNA Conformations,Nucleic Acid Conformations,RNA Conformations
D002499	Centrifugation, Density Gradient	Separation of particles according to density by employing a gradient of varying densities. At equilibrium each particle settles in the gradient at a point equal to its density. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)	Centrifugations, Density Gradient,Density Gradient Centrifugation,Density Gradient Centrifugations,Gradient Centrifugation, Density,Gradient Centrifugations, Density
D004260	DNA Repair	The removal of DNA LESIONS and/or restoration of intact DNA strands without BASE PAIR MISMATCHES, intrastrand or interstrand crosslinks, or discontinuities in the DNA sugar-phosphate backbones.	DNA Damage Response