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The nucleoid sedimentation technique is one of the most sensitive methods for measuring DNA excision repair. With this technique, we have shown that both UV- and ionizing radiation-type repair (the latter induced by bleomycin) can be discriminated in HeLa and normal diploid cells using 1-beta-D-arabinofuranosylcytosine. The latter compound inhibits UV-type repair synthesis, and thus causes DNA breaks due to enzymic incision to persist, but has no effect on rejoining DNA after ionizing radiation-type damage. It was then possible to prove that 4-nitroquinoline-1-oxide induces both types of lesions which are repaired simultaneously. This effect could be demonstrated in HeLa and normal human diploid cells in a single experimental set-up.
P Aldenhoff, and
K Sperling
March 1988,
Zhonghua yi xue za zhi,
P Aldenhoff, and
K Sperling
January 1984,
Nucleic acids symposium series,
P Aldenhoff, and
K Sperling
February 1985,
Biochemical and biophysical research communications,
P Aldenhoff, and
K Sperling
January 1989,
Yi chuan xue bao = Acta genetica Sinica,
P Aldenhoff, and
K Sperling
December 1992,
Proceedings of the National Academy of Sciences of the United States of America,
P Aldenhoff, and
K Sperling
March 2012,
Nihon rinsho. Japanese journal of clinical medicine,
P Aldenhoff, and
K Sperling
January 1986,
Basic life sciences,
P Aldenhoff, and
K Sperling
October 1996,
Seminars in radiation oncology,
P Aldenhoff, and
K Sperling
January 1983,
Environmental mutagenesis,
P Aldenhoff, and
K Sperling
February 1988,
Journal of cell science,
