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Studies of 59Fe3+ uptake by brush-border membrane vesicles prepared from mouse duodenum have indicated that uptake represents transport across the brush-border membrane which is rate-limited by the membrane-transfer step (Simpson, R.J. and Peters, T.J. (1984) Biochim. Biophys. Acta 772, 220-226). Further studies presented here reveal that the uptake rate represents the net influx rate for Fe3+ and is independent of Na+ in the medium and of the method of vesicle preparation. Uptake by brush-border membrane vesicles prepared from mouse distal ileum also represents predominantly transport and is higher than that observed with duodenal brush-border membrane vesicles. Studies of the initial uptake rate by vesicles prepared from normal and hypoxic mouse intestine demonstrated an increase in Fe3+ transport in duodenal vesicles only.
R J Simpson, and
K B Raja, and
T J Peters
September 1978,
The Biochemical journal,
R J Simpson, and
K B Raja, and
T J Peters
July 1979,
Biochimica et biophysica acta,
R J Simpson, and
K B Raja, and
T J Peters
January 1981,
Gastroenterology,
R J Simpson, and
K B Raja, and
T J Peters
April 1985,
Biochimica et biophysica acta,
R J Simpson, and
K B Raja, and
T J Peters
September 1985,
American journal of obstetrics and gynecology,
R J Simpson, and
K B Raja, and
T J Peters
May 1984,
Biochimica et biophysica acta,
R J Simpson, and
K B Raja, and
T J Peters
January 1984,
Renal physiology,
R J Simpson, and
K B Raja, and
T J Peters
March 1978,
Pflugers Archiv : European journal of physiology,
R J Simpson, and
K B Raja, and
T J Peters
December 1984,
Biochimica et biophysica acta,
R J Simpson, and
K B Raja, and
T J Peters
May 1986,
Gastroenterology,
