-transparent.png){kind=logo}
Monoclonal antibodies to bovine alpha-crystallin have been produced using hybridoma technology. Five selected hybridoma clones were maintained as ascites tumours in mice and gram quantities of the antibodies were purified from the ascites fluids by affinity chromatography on alpha m-crystallin covalently bound to Sepharose CL-2B. Preliminary characterization studies suggest that the antibodies are pure and monospecific. The antibodies could be divided into two groups on the basis of their reactivities towards iodinated alpha-crystallin, their ability to bind to Protein A-Sepharose, their immunoglobulin subclass, their immunoelectrophoretic patterns and their abilities to react with chicken and opossum alpha-crystallin. The availability of these monoclonal antibodies will greatly facilitate studies on the surface topography of alpha-crystallin.
R C Augusteyn, and
R Seifert, and
L Tai, and
C H Thompson
May 1978,
Biochimica et biophysica acta,
R C Augusteyn, and
R Seifert, and
L Tai, and
C H Thompson
November 1990,
Journal of dairy science,
R C Augusteyn, and
R Seifert, and
L Tai, and
C H Thompson
November 2009,
Molecular vision,
R C Augusteyn, and
R Seifert, and
L Tai, and
C H Thompson
October 1963,
Experimental eye research,
R C Augusteyn, and
R Seifert, and
L Tai, and
C H Thompson
October 1987,
Hybridoma,
R C Augusteyn, and
R Seifert, and
L Tai, and
C H Thompson
October 1987,
Collagen and related research,
R C Augusteyn, and
R Seifert, and
L Tai, and
C H Thompson
October 1996,
Experimental eye research,
R C Augusteyn, and
R Seifert, and
L Tai, and
C H Thompson
August 1987,
Current eye research,
R C Augusteyn, and
R Seifert, and
L Tai, and
C H Thompson
April 1988,
Experimental eye research,
R C Augusteyn, and
R Seifert, and
L Tai, and
C H Thompson
March 1984,
Current eye research,
