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Hybridoma antibodies against bovine collagenase inhibitor were produced by fusion of myeloma cells NS-1 (P3-NS1-1) with spleen cells from mice hyperimmunized with collagenase inhibitor purified from the explant medium of bovine dental pulps. Hybridomas positive by an enzyme-linked immunosorbent assay (ELISA) for bovine collagenase inhibitor were cloned by the dilution method. Seventeen hybridomas producing antibodies were isolated, four of which also recognized purified human collagenase inhibitor in the ELISA. Using a monoclonal antibody-Sepharose affinity column, we easily purified both bovine and human collagenase inhibitors to homogeneity. They showed the same mobility on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, corresponding to a molecular mass of 32,000 daltons.
S Kodama, and
J Kishi, and
K Obata, and
K Iwata, and
T Hayakawa
October 1987,
Hybridoma,
S Kodama, and
J Kishi, and
K Obata, and
K Iwata, and
T Hayakawa
January 1985,
Current eye research,
S Kodama, and
J Kishi, and
K Obata, and
K Iwata, and
T Hayakawa
August 1995,
Hybridoma,
S Kodama, and
J Kishi, and
K Obata, and
K Iwata, and
T Hayakawa
October 1989,
Biological chemistry Hoppe-Seyler,
S Kodama, and
J Kishi, and
K Obata, and
K Iwata, and
T Hayakawa
January 1986,
Animal genetics,
S Kodama, and
J Kishi, and
K Obata, and
K Iwata, and
T Hayakawa
July 1984,
Veterinary immunology and immunopathology,
S Kodama, and
J Kishi, and
K Obata, and
K Iwata, and
T Hayakawa
December 1986,
Biochemistry and cell biology = Biochimie et biologie cellulaire,
S Kodama, and
J Kishi, and
K Obata, and
K Iwata, and
T Hayakawa
June 1988,
FEBS letters,
S Kodama, and
J Kishi, and
K Obata, and
K Iwata, and
T Hayakawa
January 1989,
Experimental and clinical immunogenetics,
S Kodama, and
J Kishi, and
K Obata, and
K Iwata, and
T Hayakawa
September 1992,
The American journal of physiology,
