-transparent.png){kind=logo}
Using plasmids containing the genes for thymidine kinase (tk) and neomycin resistance (neo), we have shown that DNA-mediated genotypic transformation of L and Chinese hamster ovary (CHO) cells is increased several-fold by the presence of the sterol-binding polyene antibiotic, amphotericin. Transformation into the same host cells, using genomic DNA, was also enhanced by amphotericin. Phenotypic expression of beta-galactosidase activity of a plasmid containing the gene for the enzyme was also markedly elevated when the antibiotic was added at transfection. Other sterol-binding polyene antibiotics also showed activity in these DNA-mediated gene transfer assays.
Related Publications
K Hidaka, and
G An, and
P Ip, and
M Kuwana, and
L Siminovitch
February 1983,
Biochemical and biophysical research communications,
K Hidaka, and
G An, and
P Ip, and
M Kuwana, and
L Siminovitch
September 1981,
Somatic cell genetics,
K Hidaka, and
G An, and
P Ip, and
M Kuwana, and
L Siminovitch
January 1998,
Radiation oncology investigations,
K Hidaka, and
G An, and
P Ip, and
M Kuwana, and
L Siminovitch
January 1995,
Neoplasma,
K Hidaka, and
G An, and
P Ip, and
M Kuwana, and
L Siminovitch
January 1984,
International review of cytology,
K Hidaka, and
G An, and
P Ip, and
M Kuwana, and
L Siminovitch
December 1984,
Philosophical transactions of the Royal Society of London. Series B, Biological sciences,
K Hidaka, and
G An, and
P Ip, and
M Kuwana, and
L Siminovitch
January 1982,
Molecular & general genetics : MGG,
K Hidaka, and
G An, and
P Ip, and
M Kuwana, and
L Siminovitch
January 1980,
Annual review of genetics,
K Hidaka, and
G An, and
P Ip, and
M Kuwana, and
L Siminovitch
January 1981,
Medicina,
K Hidaka, and
G An, and
P Ip, and
M Kuwana, and
L Siminovitch
January 2004,
Methods in molecular biology (Clifton, N.J.),
