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The addition of arachidonic acid (20:4), but not other fatty acids, including the structurally similar eicosapentaenoic acid (20:5), induced specific morphological changes in cultured endothelial cells derived from bovine aorta and pulmonary artery. Cells exhibited a time- and dose-dependent change from their normal, epithelioid morphology to become elongated, polygonal, and spindle-shaped. Cells isolated from aorta appeared more sensitive to these changes than those from pulmonary artery. The effect was observed as early as 12 h after exposure to 20:4, required 48 h for maximal expression, and could be reversed in 2-5 h after change to normal media. The morphological alteration was not observed in cells treated with leukotrienes or PGE2. When cells were pretreated with ibuprofen, aspirin, or indomethacin to block prostaglandin synthesis and then exposed to 20:4, the dose-response effect was shifted to the left. This increased sensitivity to 20:4 suggests either a direct effect of 20:4 on cell morphology or an indirect effect due to metabolites of 20:4 which are not dependent on the cyclooxygenase pathway.
A Sandra, and
R S Bar, and
S Dolash, and
S J Marshall, and
T L Kaduce, and
A A Spector
April 1989,
Investigative ophthalmology & visual science,
A Sandra, and
R S Bar, and
S Dolash, and
S J Marshall, and
T L Kaduce, and
A A Spector
June 1993,
Stroke,
A Sandra, and
R S Bar, and
S Dolash, and
S J Marshall, and
T L Kaduce, and
A A Spector
January 1986,
Archives of toxicology. Supplement. = Archiv fur Toxikologie. Supplement,
A Sandra, and
R S Bar, and
S Dolash, and
S J Marshall, and
T L Kaduce, and
A A Spector
January 1983,
Arteriosclerosis (Dallas, Tex.),
A Sandra, and
R S Bar, and
S Dolash, and
S J Marshall, and
T L Kaduce, and
A A Spector
October 1992,
The American journal of physiology,
A Sandra, and
R S Bar, and
S Dolash, and
S J Marshall, and
T L Kaduce, and
A A Spector
December 1978,
Experimental and molecular pathology,
A Sandra, and
R S Bar, and
S Dolash, and
S J Marshall, and
T L Kaduce, and
A A Spector
January 1997,
The Southeast Asian journal of tropical medicine and public health,
A Sandra, and
R S Bar, and
S Dolash, and
S J Marshall, and
T L Kaduce, and
A A Spector
January 2005,
Endothelium : journal of endothelial cell research,
A Sandra, and
R S Bar, and
S Dolash, and
S J Marshall, and
T L Kaduce, and
A A Spector
July 1987,
The Biochemical journal,
A Sandra, and
R S Bar, and
S Dolash, and
S J Marshall, and
T L Kaduce, and
A A Spector
March 2019,
Biochimica et biophysica acta. Molecular and cell biology of lipids,
