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The origin of replication of Bacillus subtilis 168 trp thy dna-1 (temperature-sensitive initiation mutant) was labeled with [3H]thymidine. Analysis of labeled cells by autoradiography revealed that most of the radioactivity was associated with cell pole areas. To label the terminus, cells that had initiated were treated with chloramphenicol to inhibit cell growth and division but to allow continued DNA synthesis. These cells were then labeled with [3H]thymidine at a time when chromosome replication was nearly complete. The distribution of radioactivity was similar to that observed in origin-labeled cells. In contrast, exponentially growing cells that were labeled for a brief time at the permissive temperature showed a random distribution of radioactivity. These data indicate that the origin and terminus of replication are located at cell poles.
E M Sonnenfeld, and
A L Koch, and
R J Doyle
July 1982,
Journal of bacteriology,
E M Sonnenfeld, and
A L Koch, and
R J Doyle
January 1975,
Journal of molecular biology,
E M Sonnenfeld, and
A L Koch, and
R J Doyle
September 1984,
Journal of general microbiology,
E M Sonnenfeld, and
A L Koch, and
R J Doyle
January 1985,
Gene,
E M Sonnenfeld, and
A L Koch, and
R J Doyle
October 1987,
Nucleic acids research,
E M Sonnenfeld, and
A L Koch, and
R J Doyle
October 1974,
The Journal of general virology,
E M Sonnenfeld, and
A L Koch, and
R J Doyle
March 1983,
Proceedings of the National Academy of Sciences of the United States of America,
E M Sonnenfeld, and
A L Koch, and
R J Doyle
January 1968,
Cold Spring Harbor symposia on quantitative biology,
E M Sonnenfeld, and
A L Koch, and
R J Doyle
September 1979,
Journal of bacteriology,
E M Sonnenfeld, and
A L Koch, and
R J Doyle
September 1983,
Gene,
