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We have studied the processing of rat and human angiotensinogen precursors by microsomal membranes as a means of determining the number of asparagine-linked oligosaccharide units per angiotensinogen molecule, and thus the utilization of potential sites of N-glycosylation. Glycosylated, processed forms of angiotensinogen were isolated by chromatography on lentil lectin-Sepharose 4B. 35S-Methionine-labeled precursor and processed forms of angiotensinogen were compared with glycosylated and nonglycosylated 35S-methionine-labeled mature forms of angiotensinogen secreted by hepatoma cells, using immunoprecipitation, sodium dodecyl sulfate-polyacrylamide gel electrophoresis and autoradiography. N-Glycosylation of secreted angiotensinogen was inhibited using tunicamycin. For rat angiotensinogen, only 2 of 3 potential sites of N-glycosylation were utilized; in contrast, all 4 potential sites of N-glycosylation of human angiotensinogen were utilized. For neither rat or human angiotensinogen precursor was there any evidence for a prosequence.
D J Campbell, and
J Bouhnik, and
E Coezy, and
J Menard, and
P Corvol
May 1983,
The Journal of biological chemistry,
D J Campbell, and
J Bouhnik, and
E Coezy, and
J Menard, and
P Corvol
May 1979,
FEBS letters,
D J Campbell, and
J Bouhnik, and
E Coezy, and
J Menard, and
P Corvol
April 1979,
The Journal of dairy research,
D J Campbell, and
J Bouhnik, and
E Coezy, and
J Menard, and
P Corvol
April 1983,
The Biochemical journal,
D J Campbell, and
J Bouhnik, and
E Coezy, and
J Menard, and
P Corvol
May 1977,
Proceedings of the National Academy of Sciences of the United States of America,
D J Campbell, and
J Bouhnik, and
E Coezy, and
J Menard, and
P Corvol
July 1987,
Journal of pharmacobio-dynamics,
D J Campbell, and
J Bouhnik, and
E Coezy, and
J Menard, and
P Corvol
October 1989,
Nihon rinsho. Japanese journal of clinical medicine,
D J Campbell, and
J Bouhnik, and
E Coezy, and
J Menard, and
P Corvol
October 1978,
Biochimica et biophysica acta,
D J Campbell, and
J Bouhnik, and
E Coezy, and
J Menard, and
P Corvol
December 2004,
Molecular cell,
D J Campbell, and
J Bouhnik, and
E Coezy, and
J Menard, and
P Corvol
December 1970,
Archives internationales de physiologie et de biochimie,
