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As reported previously (Namba et al., 1985), normal human fibroblasts were transformed by 60Co gamma-ray irradiation into immortal cells with abnormal karyotypes. These transformed cells (KMST-6), however, showed a low cloning efficiency in soft agar and no transplantability. However, upon treatment with Harvey murine sarcoma virus (Ha-MSV), the cells acquired elevated clonability in soft agar and transplantability in nude mice. Ha-MSV alone, however, did not convert normal human fibroblasts into either immortal or tumorigenic cells. The Ha-MSV-transformed KMST-6 cells showed an enhanced expression of the ras oncogene, but normal and 60Co gamma-ray-transformed cells did not. Our current data suggest that gamma rays worked against normal human cells as an initiator, giving rise to chromosome aberrations and immortality, and that Ha-MSV, probably through its ras oncogene, played a role in the progression of the malignant cell population to a more malignant one showing enhanced colony formation in soft agar and tumorigenicity in nude mice.
M Namba, and
K Nishitani, and
F Fukushima, and
T Kimoto, and
K Nose
October 1993,
In vitro cellular & developmental biology. Animal,
M Namba, and
K Nishitani, and
F Fukushima, and
T Kimoto, and
K Nose
February 1992,
International journal of cancer,
M Namba, and
K Nishitani, and
F Fukushima, and
T Kimoto, and
K Nose
February 1984,
Carcinogenesis,
M Namba, and
K Nishitani, and
F Fukushima, and
T Kimoto, and
K Nose
January 1984,
The British journal of cancer. Supplement,
M Namba, and
K Nishitani, and
F Fukushima, and
T Kimoto, and
K Nose
January 1985,
Carcinogenesis; a comprehensive survey,
M Namba, and
K Nishitani, and
F Fukushima, and
T Kimoto, and
K Nose
September 1980,
Cancer research,
M Namba, and
K Nishitani, and
F Fukushima, and
T Kimoto, and
K Nose
March 1981,
The Journal of general virology,
M Namba, and
K Nishitani, and
F Fukushima, and
T Kimoto, and
K Nose
January 1987,
Experimental gerontology,
M Namba, and
K Nishitani, and
F Fukushima, and
T Kimoto, and
K Nose
May 1980,
International journal of radiation biology and related studies in physics, chemistry, and medicine,
M Namba, and
K Nishitani, and
F Fukushima, and
T Kimoto, and
K Nose
September 1982,
International journal of cancer,
