-transparent.png){kind=logo}
A new radioisotopic technique has been developed for quantification of deposition of neutrophilic granulocytes on vascular grafts. Nine healthy mongrel dogs underwent bilateral femoral artery resection and reconstruction with grafts of femoral vein and Gore-Tex. Pure granulocytes that had been separated from whole blood by centrifugal elutriation were labeled with 111In-tropolone in plasma. The granulocyte harvesting efficiency was 25 +/- 12%, and the labeling efficiency was 87 +/- 7%. Three hours after injection of labeled granulocytes and 2 hours after reperfusion, the grafts were harvested and cut into several segments for study of areas of anastomoses and midsections. On the basis of the radioactivity in the blood and in anastomotic and graft sections, the area of graft sections, and the neutrophilic granulocyte and differential leukocyte counts, the number of neutrophilic granulocytes adherent to a unit area and the total number of neutrophilic granulocytes on graft sections were calculated. These quantifications of the deposition of neutrophilic granulocytes indicated that the midsections of Gore-Tex grafts retained more neutrophilic granulocytes than did the midsections of vein grafts. Although the anastomotic areas retained more neutrophilic granulocytes than did the midsections of vein grafts, the opposite finding prevailed for the Gore-Tex grafts. A major fraction of neutrophilic granulocytes on Gore-Tex grafts was incorporated into thrombus. Semiquantitative information obtained by scintigraphy of the deposition of neutrophilic granulocytes on vascular grafts also confirmed this observation.
M K Dewanjee, and
E Solis, and
S T Mackey, and
S Socher, and
S Chowdhury, and
F P Wu, and
M P Kaye
May 1978,
Mayo Clinic proceedings,
M K Dewanjee, and
E Solis, and
S T Mackey, and
S Socher, and
S Chowdhury, and
F P Wu, and
M P Kaye
January 1986,
European journal of nuclear medicine,
M K Dewanjee, and
E Solis, and
S T Mackey, and
S Socher, and
S Chowdhury, and
F P Wu, and
M P Kaye
April 1987,
Nuklearmedizin. Nuclear medicine,
M K Dewanjee, and
E Solis, and
S T Mackey, and
S Socher, and
S Chowdhury, and
F P Wu, and
M P Kaye
January 1985,
European journal of nuclear medicine,
M K Dewanjee, and
E Solis, and
S T Mackey, and
S Socher, and
S Chowdhury, and
F P Wu, and
M P Kaye
April 1993,
Gastroenterology,
M K Dewanjee, and
E Solis, and
S T Mackey, and
S Socher, and
S Chowdhury, and
F P Wu, and
M P Kaye
June 1992,
American journal of veterinary research,
M K Dewanjee, and
E Solis, and
S T Mackey, and
S Socher, and
S Chowdhury, and
F P Wu, and
M P Kaye
January 2000,
European radiology,
M K Dewanjee, and
E Solis, and
S T Mackey, and
S Socher, and
S Chowdhury, and
F P Wu, and
M P Kaye
November 2019,
FASEB journal : official publication of the Federation of American Societies for Experimental Biology,
M K Dewanjee, and
E Solis, and
S T Mackey, and
S Socher, and
S Chowdhury, and
F P Wu, and
M P Kaye
November 1964,
Deutsche tierarztliche Wochenschrift,
M K Dewanjee, and
E Solis, and
S T Mackey, and
S Socher, and
S Chowdhury, and
F P Wu, and
M P Kaye
March 1987,
Investigative radiology,
