Rearing Culicoides obsoletus (Diptera, Ceratopogonidae) on agar cultures of nematodes. 1985

J Boorman

Culicoides obsoletus midges were reared on a culture of nematodes in small Petri dishes on an agar-based medium. At 25 C pupae were formed from 12 to 71 days after the eggs hatched. The efficiency of the method was low but, if improved, could be useful for investigating relationships between members of the Avaritia subgenus. An unexplained feature was the almost total absence of females among these laboratory-reared midges.

UI MeSH Term Description Entries
D009348 Nematoda A phylum of unsegmented helminths with fundamental bilateral symmetry and secondary triradiate symmetry of the oral and esophageal structures. Many species are parasites. Phasmidia,Secernentea,Sipunculida
D002519 Ceratopogonidae A family of biting midges, in the order DIPTERA. It includes the genus Culicoides which transmits filarial parasites pathogenic to man and other primates. Culicoides,Leptoconops,Midges, Biting,Biting Midge,Biting Midges,Culicoide,Leptoconop,Midge, Biting
D003470 Culture Media Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN. Media, Culture
D000362 Agar A complex sulfated polymer of galactose units, extracted from Gelidium cartilagineum, Gracilaria confervoides, and related red algae. It is used as a gel in the preparation of solid culture media for microorganisms, as a bulk laxative, in making emulsions, and as a supporting medium for immunodiffusion and immunoelectrophoresis.
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D000830 Animals, Laboratory Animals used or intended for use in research, testing, or teaching. Laboratory Animals,Animal, Laboratory,Laboratory Animal

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