Biomaterial Database

Rearing Culicoides variipennis sonorensis (Diptera: Ceratopogonidae) on agar and nematodes. 1994

B A Mullens, and R K Velten

Department of Entomology, University of California, Riverside 92521.

Culicoides variipennis sonorensis Wirth & Jones was reared from egg to adult in petri dishes on a 0.5 or 1% agar substrate with bacterial-feeding nematodes (Pelodera, Panagrellus) as food. All larval stages except the first instar were observed attacking and feeding on nematodes. Developmental rate on agar was approximately 25% slower than that of larvae reared in pans of water with bacteria, fungi, and algae as food. Pupation success was approximately 75% from dishes (3.5 or 9 cm diameter) with 30 and 100 eggs, respectively. The technique has significant advantages for maintainance of small laboratory colonies and allows continuous observation of developing immatures for experimental studies.

UI	MeSH Term	Description	Entries
D007814	Larva	Wormlike or grublike stage, following the egg in the life cycle of insects, worms, and other metamorphosing animals.	Maggots,Tadpoles,Larvae,Maggot,Tadpole
D009348	Nematoda	A phylum of unsegmented helminths with fundamental bilateral symmetry and secondary triradiate symmetry of the oral and esophageal structures. Many species are parasites.	Phasmidia,Secernentea,Sipunculida
D002519	Ceratopogonidae	A family of biting midges, in the order DIPTERA. It includes the genus Culicoides which transmits filarial parasites pathogenic to man and other primates.	Culicoides,Leptoconops,Midges, Biting,Biting Midge,Biting Midges,Culicoide,Leptoconop,Midge, Biting
D004435	Eating	The consumption of edible substances.	Dietary Intake,Feed Intake,Food Intake,Macronutrient Intake,Micronutrient Intake,Nutrient Intake,Nutritional Intake,Ingestion,Dietary Intakes,Feed Intakes,Intake, Dietary,Intake, Feed,Intake, Food,Intake, Macronutrient,Intake, Micronutrient,Intake, Nutrient,Intake, Nutritional,Macronutrient Intakes,Micronutrient Intakes,Nutrient Intakes,Nutritional Intakes
D004772	Entomology	A discipline or occupation concerned with the study of INSECTS, including the biology and the control of insects.
D000362	Agar	A complex sulfated polymer of galactose units, extracted from Gelidium cartilagineum, Gracilaria confervoides, and related red algae. It is used as a gel in the preparation of solid culture media for microorganisms, as a bulk laxative, in making emulsions, and as a supporting medium for immunodiffusion and immunoelectrophoresis.
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia