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Using primary cultures of parenchymal hepatocytes as a model system, the cytotoxic potential of dantrolene sodium (DS) was compared with that of erythromycin estolate (EE)--a known hepatotoxin. Parallel morphological and functional comparisons were made, following 4-, 8-, or 24-h exposures of hepatocyte cultures, using phase contrast microscopy and lactate dehydrogenase (LDH) leakage, respectively. Four-hour exposures of cultures to rather low concentrations of EE (i.e. 50 microM) resulted in cellular necrosis and significantly elevated LDH release. As the concentration of this hepatotoxin was increased, the changes were more pronounced. However, even 4- or 8-h exposures of cultures to a maximum of 100 microM DS did not affect LDH leakage or morphological integrity, although marginally detectable morphological changes did not occur at the highest concentration after 24-h. The value of using primary parenchymal hepatocyte cultures as a model system for the assessment of xenobiotic-induced hepatotoxicity was confirmed.
E M Sorensen, and
D Acosta
January 1985,
Xenobiotica; the fate of foreign compounds in biological systems,
E M Sorensen, and
D Acosta
February 1987,
In vitro cellular & developmental biology : journal of the Tissue Culture Association,
E M Sorensen, and
D Acosta
January 1990,
Methods in molecular biology (Clifton, N.J.),
E M Sorensen, and
D Acosta
September 1977,
JAMA,
E M Sorensen, and
D Acosta
March 1992,
The Pediatric infectious disease journal,
E M Sorensen, and
D Acosta
June 1962,
Antibiotics & chemotherapy (Northfield, Ill.),
E M Sorensen, and
D Acosta
December 1971,
The Medical journal of Australia,
E M Sorensen, and
D Acosta
April 1972,
Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.),
E M Sorensen, and
D Acosta
January 2006,
Methods in molecular biology (Clifton, N.J.),
E M Sorensen, and
D Acosta
January 2006,
Methods in molecular biology (Clifton, N.J.),
