-transparent.png){kind=logo}
An effective preparative isoelectric focusing method has been developed using the LKB Immobiline system in a vertical slab gel apparatus. Advantages of this procedure are ease of sample application, excellent resolution, and the direct visualization of focused bands. Narrow pH gradients have been used to separate apolipoprotein E3 isoforms (pH gradient 4.9-5.9) and to resolve the apolipoprotein C mixture (pH gradient 4.0-5.0). Recoveries ranged from 40 to 70%. The method should be valuable for protein and isoform purification.
Related Publications
K H Weisgraber, and
Y M Newhouse, and
J L Seymour, and
S C Rall, and
R W Mahley
November 1992,
American biotechnology laboratory,
K H Weisgraber, and
Y M Newhouse, and
J L Seymour, and
S C Rall, and
R W Mahley
January 1987,
Journal of biochemical and biophysical methods,
K H Weisgraber, and
Y M Newhouse, and
J L Seymour, and
S C Rall, and
R W Mahley
March 1975,
Biochimica et biophysica acta,
K H Weisgraber, and
Y M Newhouse, and
J L Seymour, and
S C Rall, and
R W Mahley
May 2001,
Current protocols in protein science,
K H Weisgraber, and
Y M Newhouse, and
J L Seymour, and
S C Rall, and
R W Mahley
January 1988,
Journal of biochemical and biophysical methods,
K H Weisgraber, and
Y M Newhouse, and
J L Seymour, and
S C Rall, and
R W Mahley
April 1988,
Clinica chimica acta; international journal of clinical chemistry,
K H Weisgraber, and
Y M Newhouse, and
J L Seymour, and
S C Rall, and
R W Mahley
November 1990,
Electrophoresis,
K H Weisgraber, and
Y M Newhouse, and
J L Seymour, and
S C Rall, and
R W Mahley
January 1988,
Journal of biochemical and biophysical methods,
K H Weisgraber, and
Y M Newhouse, and
J L Seymour, and
S C Rall, and
R W Mahley
January 1982,
Human genetics,
K H Weisgraber, and
Y M Newhouse, and
J L Seymour, and
S C Rall, and
R W Mahley
November 1984,
Analytical biochemistry,
