Biomaterial Database

Capping of ricin-binding sites does not influence phagocytosis in human polymorphonuclear leukocytes. 1978

M E Feigenson, and M Baggiolini

Human polymorphonuclear leukocytes (PMN'S) were incubated at 0 degrees C with ferritin-conjugated ricin and warmed to 37 degrees C to induce capping of the ricin-binding sites. The PMNs were then allowed to phagocytose yeast or Staphylococcus epidermidis for 15 min, and processed for electron microscopy. Phagocytic uptake, granule fusion, and the fate of lectin-bound membrane were quantified by morphometry. Ricin-capped PMNs phagocytosed as extensively as untreated PMNs. Particles were ingested almost exclusively with a lectin-free portion of the plasmalemma. Fusion of granules with phagocytic vacuoles was not affected by ricin-induced capping. This indicates that ricin-binding sites are not involved in particle recognition and uptake.

UI	MeSH Term	Description	Entries
D007962	Leukocytes	White blood cells. These include granular leukocytes (BASOPHILS; EOSINOPHILS; and NEUTROPHILS) as well as non-granular leukocytes (LYMPHOCYTES and MONOCYTES).	Blood Cells, White,Blood Corpuscles, White,White Blood Cells,White Blood Corpuscles,Blood Cell, White,Blood Corpuscle, White,Corpuscle, White Blood,Corpuscles, White Blood,Leukocyte,White Blood Cell,White Blood Corpuscle
D010587	Phagocytosis	The engulfing and degradation of microorganisms; other cells that are dead, dying, or pathogenic; and foreign particles by phagocytic cells (PHAGOCYTES).	Phagocytoses
D002462	Cell Membrane	The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.	Plasma Membrane,Cytoplasmic Membrane,Cell Membranes,Cytoplasmic Membranes,Membrane, Cell,Membrane, Cytoplasmic,Membrane, Plasma,Membranes, Cell,Membranes, Cytoplasmic,Membranes, Plasma,Plasma Membranes
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D001665	Binding Sites	The parts of a macromolecule that directly participate in its specific combination with another molecule.	Combining Site,Binding Site,Combining Sites,Site, Binding,Site, Combining,Sites, Binding,Sites, Combining
D012276	Ricin	A protein phytotoxin from the seeds of Ricinus communis, the castor oil plant. It agglutinates cells, is proteolytic, and causes lethal inflammation and hemorrhage if taken internally.	Castor Bean Lectin,Lectin, Castor Bean,Lectin, Ricinus,Ricin Toxin,RCA 60,RCA60,Ricin A Chain,Ricin B Chain,Ricin D,Ricin I,Ricinus Toxin,A Chain, Ricin,B Chain, Ricin,Ricinus Lectin,Toxin, Ricin,Toxin, Ricinus