The non-metabolizable glucose analogue 2-deoxy-d-glucose is taken up by Pseudomonas aeruginosa against a concentration gradient, in a predominantly unchanged form. d-Glucose competitively inhibits 2-deoxy-d-glucose uptake and also causes a rapid exit of intracellular 2-deoxy-d-glucose. Thus these two sugars share the same stereospecific carrier system, and glucose transport can be studied reliably with 2-deoxy-d-glucose. The transport system is inducible, and is strongly repressed by a number of organic acids such as acetate, citrate, succinate, fumarate and malate, even in the presence of adequate excess of the inducer (d-glucose). Repression by organic acids can be relieved by transferring cells to a glucose medium, but in the presence of chloramphenicol the cells fail to recover from repression, indicating that the formation of the transport system involves the synthesis of protein. The results demonstrate that the regulation of glucose metabolism effected by citric acid-cycle intermediates in P. aeruginosa is manifest at the level of the glucose-transport system.