-transparent.png){kind=logo}
Isatin has been found to inhibit rat kidney alkaline phosphatase (EC 3.1.3.1). The inhibition is dependent on isatin concentration and is of un-competitive type. The hydrolysis of disodium phenyl phosphate by the enzyme at different temperatures (17--37 degrees C) obeys the Arrhenius equation. Energy of activation in the absence and presence of isatin has been found to be 9.84 and 10.24 kCal/mol. The hyperbolic profile of isatin inhibition; the lowering of both Km and Vmax in the presence of isatin, and, small changes in enthalpy, free energy and entropy in the presence of isatin suggest a non-allosteric un-competitive inhibition of the enzyme.
Related Publications
B Singh, and
R Sharma, and
J P Nagpaul, and
R C Bansal, and
P Kumar
January 1979,
Enzyme,
B Singh, and
R Sharma, and
J P Nagpaul, and
R C Bansal, and
P Kumar
December 1969,
Enzymologia,
B Singh, and
R Sharma, and
J P Nagpaul, and
R C Bansal, and
P Kumar
November 1967,
Archives of biochemistry and biophysics,
B Singh, and
R Sharma, and
J P Nagpaul, and
R C Bansal, and
P Kumar
October 1964,
Nature,
B Singh, and
R Sharma, and
J P Nagpaul, and
R C Bansal, and
P Kumar
March 1975,
Biochimica et biophysica acta,
B Singh, and
R Sharma, and
J P Nagpaul, and
R C Bansal, and
P Kumar
January 1958,
Archives of biochemistry and biophysics,
B Singh, and
R Sharma, and
J P Nagpaul, and
R C Bansal, and
P Kumar
January 1989,
Acta diabetologica latina,
B Singh, and
R Sharma, and
J P Nagpaul, and
R C Bansal, and
P Kumar
March 1950,
Comptes rendus des seances de la Societe de biologie et de ses filiales,
B Singh, and
R Sharma, and
J P Nagpaul, and
R C Bansal, and
P Kumar
April 1967,
Biochimica et biophysica acta,
B Singh, and
R Sharma, and
J P Nagpaul, and
R C Bansal, and
P Kumar
February 1976,
Journal of biochemistry,
