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The components of the hemoglobin-A1 fraction--hemoglobins A1a--c--arise from nonenzymatic glycosylation of hemoglobin A at the beta-chain N-terminal amino groups and can be resolved from hemoglobin A by cation exchange chromatography. Glycosylation can also occur at the alpha-chain N-terminals as well as the epsilon-amino groups of lysine residues of both alpha- and beta-chains; this results in glycosylated species appearing in the hemoglobin-A fraction. In this study, we determined the extent of hemoglobin-A glycosylation using a colorimetric chemical method specific for the detection of ketoamine-linked hexoses in proteins. We demonstrate increased glycosylation of the main hemoglobin-A fraction in diabetic patients, which correlates significantly (r = 0.72, P less than 0.001) with the hemoglobin-A1 percentage determined by column chromatography in the corresponding hemolysates. This finding provides the basis for the application of this chemical procedure to the measurement of total glycosylation of hemoglobin.
K H Gabbay, and
J M Sosenko, and
G A Banuchi, and
M J Mininsohn, and
R Flückiger
July 1981,
Diabetes,
K H Gabbay, and
J M Sosenko, and
G A Banuchi, and
M J Mininsohn, and
R Flückiger
January 1986,
Revista de la Facultad de Ciencias Medicas (Cordoba, Argentina),
K H Gabbay, and
J M Sosenko, and
G A Banuchi, and
M J Mininsohn, and
R Flückiger
April 1983,
Journal of the National Medical Association,
K H Gabbay, and
J M Sosenko, and
G A Banuchi, and
M J Mininsohn, and
R Flückiger
March 1981,
Drug intelligence & clinical pharmacy,
K H Gabbay, and
J M Sosenko, and
G A Banuchi, and
M J Mininsohn, and
R Flückiger
January 1979,
Acta diabetologica latina,
K H Gabbay, and
J M Sosenko, and
G A Banuchi, and
M J Mininsohn, and
R Flückiger
January 1979,
La Nouvelle presse medicale,
K H Gabbay, and
J M Sosenko, and
G A Banuchi, and
M J Mininsohn, and
R Flückiger
February 1980,
Clinical chemistry,
K H Gabbay, and
J M Sosenko, and
G A Banuchi, and
M J Mininsohn, and
R Flückiger
January 1987,
Clinical physiology and biochemistry,
K H Gabbay, and
J M Sosenko, and
G A Banuchi, and
M J Mininsohn, and
R Flückiger
April 1981,
Taiwan yi xue hui za zhi. Journal of the Formosan Medical Association,
K H Gabbay, and
J M Sosenko, and
G A Banuchi, and
M J Mininsohn, and
R Flückiger
October 1982,
Psychosomatics,
