-transparent.png){kind=logo}
Globin messenger RNA (mRNA) isolated from three patients homozygous for hemoglobin Lepore is shown to have a marked reduction of the amount of beta-like globin mRNA (Lepore-globin mRNA sequences) compared with alpha-globin mRNA by molecular hybridization. The relative amounts of alpha- and Lepore mRNA are similar to the amounts of alpha- and Lepore globin synthesized in intact cells and by isolated mRNA in a cell-free system. It is also demonstrated that Lepore-globin mRNA can completely hybridize to full-length or nearly full-length beta-globin specific complementary DNA and protect it from nuclease digestion, indicating close homology between the delta-mRNA sequences present in Lepore mRNA and the beta-complementary-DNA probe. We have also quantitated the numbers of beta-like globin gene sequences in genomic Lepore DNA by molecular hybridization and demonstrated a reduction in their number consistent with the Lepore gene being a delta beta-gene fusion product.
F Ramirez, and
J G Mears, and
U Nudel, and
A Bank, and
L Luzzatto, and
G DiPrisco, and
R D'Avino, and
G Pepe, and
L Camardella, and
R Gambino, and
R Cimino, and
N Quattrin
April 1979,
European journal of biochemistry,
F Ramirez, and
J G Mears, and
U Nudel, and
A Bank, and
L Luzzatto, and
G DiPrisco, and
R D'Avino, and
G Pepe, and
L Camardella, and
R Gambino, and
R Cimino, and
N Quattrin
December 1973,
Blood,
F Ramirez, and
J G Mears, and
U Nudel, and
A Bank, and
L Luzzatto, and
G DiPrisco, and
R D'Avino, and
G Pepe, and
L Camardella, and
R Gambino, and
R Cimino, and
N Quattrin
August 1970,
Biochemical and biophysical research communications,
F Ramirez, and
J G Mears, and
U Nudel, and
A Bank, and
L Luzzatto, and
G DiPrisco, and
R D'Avino, and
G Pepe, and
L Camardella, and
R Gambino, and
R Cimino, and
N Quattrin
January 1971,
Series haematologica (1968),
F Ramirez, and
J G Mears, and
U Nudel, and
A Bank, and
L Luzzatto, and
G DiPrisco, and
R D'Avino, and
G Pepe, and
L Camardella, and
R Gambino, and
R Cimino, and
N Quattrin
March 1976,
Cell,
F Ramirez, and
J G Mears, and
U Nudel, and
A Bank, and
L Luzzatto, and
G DiPrisco, and
R D'Avino, and
G Pepe, and
L Camardella, and
R Gambino, and
R Cimino, and
N Quattrin
January 1978,
Hemoglobin,
F Ramirez, and
J G Mears, and
U Nudel, and
A Bank, and
L Luzzatto, and
G DiPrisco, and
R D'Avino, and
G Pepe, and
L Camardella, and
R Gambino, and
R Cimino, and
N Quattrin
April 1982,
The Journal of clinical investigation,
F Ramirez, and
J G Mears, and
U Nudel, and
A Bank, and
L Luzzatto, and
G DiPrisco, and
R D'Avino, and
G Pepe, and
L Camardella, and
R Gambino, and
R Cimino, and
N Quattrin
November 1972,
Science (New York, N.Y.),
F Ramirez, and
J G Mears, and
U Nudel, and
A Bank, and
L Luzzatto, and
G DiPrisco, and
R D'Avino, and
G Pepe, and
L Camardella, and
R Gambino, and
R Cimino, and
N Quattrin
December 1981,
The Journal of clinical investigation,
F Ramirez, and
J G Mears, and
U Nudel, and
A Bank, and
L Luzzatto, and
G DiPrisco, and
R D'Avino, and
G Pepe, and
L Camardella, and
R Gambino, and
R Cimino, and
N Quattrin
January 1975,
Biokhimiia (Moscow, Russia),
