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Papain-solubilized HLA-A, -B, and -C antigens have been isolated from cadaveric spleens. The isolated material was homogeneous and comprised subunits with the apparent molecular weights 33,000 and 12,000. Amino acid analyses of a mixture of HLA antigen heavy chains obtained from a great number of spleens with different HLA antigen phenotypes revealed a composition that is very similar to that of individual HLA-A and -B antigens. Likewise, the NH2-terminal 30 residues of the HLA-antigen heavy chain mixture were virtually identical with that recorded for individual specificities. The circular dichroism spectra for the isolated HLA antigens and for free beta2-microglobulin revealed similarities with spectra recorded for immunoglobulin chains and domains. The HLA-antigen heavy chain may contain an appreciable amount of beta structure. Antibodies raised against free beta2-microglobulin react better with beta2-microglobulin in free form than when bound to the HLA-A, -B, and -C antigen heavy chains. This is due to the fact that free beta2-microglobulin can bind a maximum of four Fab fragments simultaneously, whereas the HLA-antigen-associated beta2-microglobulin can bind only two Fab fragments without dissociating from the heavy HLA-antigen subunit.
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B Curman, and
K Wiman, and
L Rask, and
P A Peterson
April 1975,
Proceedings of the National Academy of Sciences of the United States of America,
L Trägårdh, and
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May 1979,
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L Trägårdh, and
B Curman, and
K Wiman, and
L Rask, and
P A Peterson
August 1979,
The Journal of biological chemistry,
L Trägårdh, and
B Curman, and
K Wiman, and
L Rask, and
P A Peterson
January 1970,
Federation proceedings,
L Trägårdh, and
B Curman, and
K Wiman, and
L Rask, and
P A Peterson
March 1971,
Transplantation proceedings,
L Trägårdh, and
B Curman, and
K Wiman, and
L Rask, and
P A Peterson
January 1980,
Scandinavian journal of immunology,
L Trägårdh, and
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K Wiman, and
L Rask, and
P A Peterson
January 1980,
Journal of immunological methods,
L Trägårdh, and
B Curman, and
K Wiman, and
L Rask, and
P A Peterson
May 1973,
The Journal of experimental medicine,
L Trägårdh, and
B Curman, and
K Wiman, and
L Rask, and
P A Peterson
March 1976,
European journal of immunology,
L Trägårdh, and
B Curman, and
K Wiman, and
L Rask, and
P A Peterson
May 1973,
Proceedings of the National Academy of Sciences of the United States of America,
