Biomaterial Database

Total substitution of bromodeoxyuridine for thymidine in the DNA of a bromodeoxyuridine-dependent cell line. 1974

M D Bick, and R L Davidson

In an attempt to isolate cells that could survive with total replacement of thymidine by bromodeoxyuridine in nuclear DNA, cells of a bromodeoxyuridine-dependent Syrian hamster line were cultured in medium containing aminopterin and bromodeoxyuridine but no thymidine. A line of cells, called HAB, was isolated. The HAB cells have been maintained in continuous cultivation for over nine months and have undergone more than 125 population doublings. Direct base analysis showed that the level of substitution of bromodeoxyuridine for thymidine in nuclear DNA was at least 99.8%, and possibly 100%. The existence of such cells raises many questions. The expected high frequency of bromodeoxyuridine-induced base transitions, including errors in both replication and transcription, would seem to be incompatible with the apparently stable transmission and expression of the genetic information in these cells.

UI	MeSH Term	Description	Entries
D008545	Melanoma	A malignant neoplasm derived from cells that are capable of forming melanin, which may occur in the skin of any part of the body, in the eye, or, rarely, in the mucous membranes of the genitalia, anus, oral cavity, or other sites. It occurs mostly in adults and may originate de novo or from a pigmented nevus or malignant lentigo. Melanomas frequently metastasize widely, and the regional lymph nodes, liver, lungs, and brain are likely to be involved. The incidence of malignant skin melanomas is rising rapidly in all parts of the world. (Stedman, 25th ed; from Rook et al., Textbook of Dermatology, 4th ed, p2445)	Malignant Melanoma,Malignant Melanomas,Melanoma, Malignant,Melanomas,Melanomas, Malignant
D008722	Methods	A series of steps taken in order to conduct research.	Techniques,Methodological Studies,Methodological Study,Procedures,Studies, Methodological,Study, Methodological,Method,Procedure,Technique
D001973	Bromodeoxyuridine	A nucleoside that substitutes for thymidine in DNA and thus acts as an antimetabolite. It causes breaks in chromosomes and has been proposed as an antiviral and antineoplastic agent. It has been given orphan drug status for use in the treatment of primary brain tumors.	BUdR,BrdU,Bromouracil Deoxyriboside,Broxuridine,5-Bromo-2'-deoxyuridine,5-Bromodeoxyuridine,NSC-38297,5 Bromo 2' deoxyuridine,5 Bromodeoxyuridine,Deoxyriboside, Bromouracil
D002460	Cell Line	Established cell cultures that have the potential to propagate indefinitely.	Cell Lines,Line, Cell,Lines, Cell
D002621	Chemistry	A basic science concerned with the composition, structure, and properties of matter; and the reactions that occur between substances and the associated energy exchange.
D004273	DNA, Neoplasm	DNA present in neoplastic tissue.	Neoplasm DNA
D004586	Electrophoresis	An electrochemical process in which macromolecules or colloidal particles with a net electric charge migrate in a solution under the influence of an electric current.	Electrophoreses
D006224	Cricetinae	A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.	Cricetus,Hamsters,Hamster
D000630	Aminopterin	A folic acid derivative used as a rodenticide that has been shown to be teratogenic.	Aminopterin Sodium,Aminopterin, Disodium Salt,Disodium Salt Aminopterin,Sodium, Aminopterin
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia