Biomaterial Database

Intracellular recording from beating heart in situ using a special micropipette holder. 1979

T Akiyama, and P Serrino

Use of a motion-compensated micropipette holder, which senses cardiac motion and lifts and lowers the micropipette and supporting apparatus in synchrony with the heart beat, allows for stable recording of transmembrane action potentials from subepicardial cells of an in vivo beating heart in an open-chest dog without significantly impairing cardiac hemodynamics. This technique may be used to study the effects of ischemia, hypertrophy, or pharmacologic agents on the cellular electrophysiological parameters of subepicardial ventricular cells of an in vivo beating heart.

UI	MeSH Term	Description	Entries
D008839	Microelectrodes	Electrodes with an extremely small tip, used in a voltage clamp or other apparatus to stimulate or record bioelectric potentials of single cells intracellularly or extracellularly. (Dorland, 28th ed)	Electrodes, Miniaturized,Electrode, Miniaturized,Microelectrode,Miniaturized Electrode,Miniaturized Electrodes
D004285	Dogs	The domestic dog, Canis familiaris, comprising about 400 breeds, of the carnivore family CANIDAE. They are worldwide in distribution and live in association with people. (Walker's Mammals of the World, 5th ed, p1065)	Canis familiaris,Dog
D004594	Electrophysiology	The study of the generation and behavior of electrical charges in living organisms particularly the nervous system and the effects of electricity on living organisms.
D006321	Heart	The hollow, muscular organ that maintains the circulation of the blood.	Hearts
D000200	Action Potentials	Abrupt changes in the membrane potential that sweep along the CELL MEMBRANE of excitable cells in response to excitation stimuli.	Spike Potentials,Nerve Impulses,Action Potential,Impulse, Nerve,Impulses, Nerve,Nerve Impulse,Potential, Action,Potential, Spike,Potentials, Action,Potentials, Spike,Spike Potential
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia