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We compare a column-chromatographic method and a homogeneous immunoassay method for separately measuring the mitochondrial and cytoplasmic isoenzymes of aspartate aminotransferase. Analytical recovery for the two methods averaged 102% (SD, 2%) and 103% (SD, 4%), respectively, for 11 pools prepared by adding the purified isoenzymes to serum and 102% (SD 8.9%) and 89% (SD, 8.1%) for 26 unaltered specimens of human serum. In comparing the results of the immunoassay method (y) to the chromatographic method (x), our measurements agreed closely for the mitochondrial (y = 0.947 X + 7, r = 0.9991, standard error of estimate = 2.9 U/L) and cytoplasmic (y = 0.92x-6, r = 0.9995, standard error of estimate = 2.1 U/L) isoenzymes in pools prepared from the purified isoenzymes. Similar measurements of the 26 human serum specimens yielded the following results for least-squares evaluation; cytoplasmic isoenzyme y = 1.03x-11, r = 0.994, and standard error of estimate = 6.0 U/L; mitochondrial isoenzyme y = 0.75x+0, r = 0.927, and standard error of estimate = 3.9 U/L.
E J Sampson, and
W H Hannon, and
S S McKneally, and
C McKenzie, and
S A Miller, and
V S Whitner, and
C A Burtis
April 1981,
Clinical chemistry,
E J Sampson, and
W H Hannon, and
S S McKneally, and
C McKenzie, and
S A Miller, and
V S Whitner, and
C A Burtis
August 1984,
Clinical chemistry,
E J Sampson, and
W H Hannon, and
S S McKneally, and
C McKenzie, and
S A Miller, and
V S Whitner, and
C A Burtis
August 1990,
Clinical biochemistry,
E J Sampson, and
W H Hannon, and
S S McKneally, and
C McKenzie, and
S A Miller, and
V S Whitner, and
C A Burtis
February 1983,
Clinica chimica acta; international journal of clinical chemistry,
E J Sampson, and
W H Hannon, and
S S McKneally, and
C McKenzie, and
S A Miller, and
V S Whitner, and
C A Burtis
January 1975,
Laboratornoe delo,
E J Sampson, and
W H Hannon, and
S S McKneally, and
C McKenzie, and
S A Miller, and
V S Whitner, and
C A Burtis
March 1972,
Quaderni Sclavo di diagnostica clinica e di laboratorio,
E J Sampson, and
W H Hannon, and
S S McKneally, and
C McKenzie, and
S A Miller, and
V S Whitner, and
C A Burtis
January 1971,
Enzyme,
E J Sampson, and
W H Hannon, and
S S McKneally, and
C McKenzie, and
S A Miller, and
V S Whitner, and
C A Burtis
October 1982,
Equine veterinary journal,
E J Sampson, and
W H Hannon, and
S S McKneally, and
C McKenzie, and
S A Miller, and
V S Whitner, and
C A Burtis
January 1971,
Enzyme,
E J Sampson, and
W H Hannon, and
S S McKneally, and
C McKenzie, and
S A Miller, and
V S Whitner, and
C A Burtis
October 1987,
Clinical chemistry,
